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WEPRO7240H series Dialysis Core Kit

Porducts

Ctandard Kit for scale-up synthesis

Protein synthesis Kit

 

WEPRO7240H series Dialysis Core Kit


 

 

 

WEPRO7240H series Dialysis Core Kit

This kit contains the reagents necessary for wheat cell-free protein synthesis by the dialysis reaction method. The yield per unit of WEPRO can be expected to increase dramatically compared to the bilayer reaction, especially when highly soluble proteins are synthesized.

Features:

  • Can be used for 2 reactions on 3ml reaction scale and 60 reactions on 50 μl reaction scale.
  • Various reaction scales are possible depending on the reaction device.

Recommended for:

  • Users who wish to synthesize a large amount of target proteins in a single reaction.
  • Users who wish to synthesize target proteins more efficiently and reduce costs associated with synthesis.
  • Users who wish to advance assays in the same lot by synthesizing a large amount.

 

* This kit does not come with a dialysis device included.

* There are a variety of dialysis devices on the market. Follow the instructions for use in the respective manuals. We are using the separate Slide-A-LyzerTM MINI Dialysis Devices, 10K MWCO (Thermo Fisher Scientific Inc.).

Flow of operations

WEPRO7240 series Dialysis Core Kit

Specification

Product Number Product Name Size   Instruction manual Safety Data Sheet
CFS-D7 WEPRO7240 Dialysis Core Kit   1 set     Download
Product Composition
 

Reagent Name

Concentration

Volume

Quantity

Transcription reaction reagents

SP6 RNA Polymerase

80 U/μl

15μl

1

RNase Inhibitor

80 U/μl

15μl

1

NTP Mix

25mM

120μl

1

5xTranscription Buffer LM

5x

120μl

1

Translation reaction reagents

WEPRO®7240

240OD

1 ml

1

40xSUB-AMIX® SGC S-1

40x

600μl

1

40xSUB-AMIX® SGC S-2

40x

600μl

1

40xSUB-AMIX® SGC S-3

40x

600μl

1

40xSUB-AMIX® SGC S-4

40x

600μl

1

Transcription reaction reagents set

Reagent Name

Concentration

Volume

Quantity

SP6 RNA Polymerase

80 U/μl

15μl

1

RNase Inhibitor

80 U/μl

15μl

1

NTP Mix

25mM

120μl

1

5xTranscription Buffer LM

5x

240μl

1

Translation reaction reagent (For external dialysis solution)

Reagent Name

Concentration

Volume

Quantity

40xSUB-AMIX® SGC S-1

40x

7ml

1

40xSUB-AMIX® SGC S-2

40x

7ml

1

40xSUB-AMIX® SGC S-3

40x

7ml

1

40xSUB-AMIX® SGC S-4

40x

7ml

1

Storage Condition

 -80 ℃

 

*SUB-AMIX SGC S1-S4 each vary in their composition.

 When producing a 1x concentration, adjust by adding the same quantity of each.

 

Product Number

Product Name

Size

CFS-D7G

WEPRO7240G Dialysis Core Kit

1 set

 

 

 

Product Composition

Reagent Name

Concentration

Volume

Quantity

Transcription reaction reagents

SP6 RNA Polymerase

80 U/μl

15μl

1

RNase Inhibitor

80 U/μl

15μl

1

NTP Mix

25mM

120μl

1

5xTranscription Buffer LM

5x

120μl

1

Translation reaction reagents

WEPRO®7240

240OD

1 ml

1

40xSUB-AMIX® SGC S-1

40x

600μl

1

40xSUB-AMIX® SGC S-2

40x

600μl

1

40xSUB-AMIX® SGC S-3

40x

600μl

1

40xSUB-AMIX® SGC S-4

40x

600μl

1

Transcription reaction reagents set

Reagent Name

Concentration

Volume

Quantity

SP6 RNA Polymerase

80 U/μl

15μl

1

RNase Inhibitor

80 U/μl

15μl

1

NTP Mix

25mM

120μl

1

5xTranscription Buffer LM

5x

240μl

1

Translation reaction reagent (For external dialysis solution)

Reagent Name

Concentration

Volume

Quantity

40xSUB-AMIX® SGC S-1

40x

7ml

1

40xSUB-AMIX® SGC S-2

40x

7ml

1

40xSUB-AMIX® SGC S-3

40x

7ml

1

40xSUB-AMIX® SGC S-4

40x

7ml

1

Storage Condition

 -80 ℃

 

*SUB-AMIX SGC S1-S4 each vary in their composition.

 When producing a 1x concentration, adjust by adding the same quantity of each.

 

Product Number

Product Name

Size

CFS-D7H

WEPRO7240H Dialysis Core Kit

1 set

 

 

 

Product Composition

Reagent Name

Concentration

Volume

Quantity

Transcription reaction reagents

SP6 RNA Polymerase

80 U/μl

15μl

1

RNase Inhibitor

80 U/μl

15μl

1

NTP Mix

25mM

120μl

1

5xTranscription Buffer LM

5x

120μl

1

Translation reaction reagents

WEPRO®7240

240OD

1 ml

1

40xSUB-AMIX® SGC S-1

40x

600μl

1

40xSUB-AMIX® SGC S-2

40x

600μl

1

40xSUB-AMIX® SGC S-3

40x

600μl

1

40xSUB-AMIX® SGC S-4

40x

600μl

1

Transcription reaction reagents set

Reagent Name

Concentration

Volume

Quantity

SP6 RNA Polymerase

80 U/μl

15μl

1

RNase Inhibitor

80 U/μl

15μl

1

NTP Mix

25mM

120μl

1

5xTranscription Buffer LM

5x

240μl

1

Translation reaction reagent (For external dialysis solution)

Reagent Name

Concentration

Volume

Quantity

40xSUB-AMIX® SGC S-1

40x

7ml

1

40xSUB-AMIX® SGC S-2

40x

7ml

1

40xSUB-AMIX® SGC S-3

40x

7ml

1

40xSUB-AMIX® SGC S-4

40x

7ml

1

Storage Condition

 -80 ℃

 

*SUB-AMIX SGC S1-S4 each vary in their composition.

 When producing a 1x concentration, adjust by adding the same quantity of each.