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RNase III, E.coli
RNase III, E.coli
| CAT.NO | UNIT |
|---|---|
| E020 | 100 U (100 μl) |
| Description | RNase III, E.coli is an endoribonuclease that specifically digests dsRNA into dsRNA fragments of 12-15 base pairs that have two or three nucleotide 3′-overhangs with 5' phosphate and 3' hydroxyl termini. |
|---|---|
| SKU | E020 |
| Unit quantity | 100 U (100 μl) |
| Applications | • dsRNA digestion to short dsRNA fragments • Useful for RNA processing, maturation and structure studies • Directed cleavage of RNA • siRNA preparation for RNA interference in mammalian cells Note: This product is not suitable for NGS or second strand cDNA synthesis applications. |
| Concentration | 1 U/μl |
| Format | Enzyme supplied with 10X Reaction Buffer |
| Storage Condition | Store at -20°C. |
| Storage Buffer | 50 mM Tris-HCl (pH 7.5), 0.5M NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton® X-100 and 50% (v/v) Glycerol. |
| Note | One unit is defined as the amount of RNase III, E. coli that is required to hydrolyze 1 nmol of poly(A):poly(U) dsRNA in 30 minutes at 37°C in 1X RNase III, E.coli Reaction Buffer.
This product is distributed for laboratory research only. Caution: Not for diagnostic use. |
Supporting Protocol
