RNase H, E. coli
CAT.NO | UNIT |
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E018 | 500 U (100 μl) |
Description | RNase H (Ribonuclease H), E. coli is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA strands in RNA-DNA hybrids. This enzyme cleaves the 3'-O-P-bond of RNA and generates 3' hydroxyl and 5' phosphate products. RNase H does not digest single-stranded or double-stranded DNA and RNA. |
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SKU | E018 |
Unit quantity | 500 U (100 μl) |
Applications |
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Concentration | 5 U/μl |
Format | Enzyme supplied with 10X Reaction Buffer |
Storage Condition | Store all components at -20°C. |
Storage Buffer | 20 mM Tris-HCl (pH 7.5), 100 mM KCl, 10 mM MgCl2, 0.1 mM DTT, 0.1 mM EDTA, and 50% (v/v) Glycerol. |
Note | One unit is defined as the amount of RNase H, E. coli that is required to hydrolyze 1 nmol of the RNA in radiolabeled poly(rA):poly(dT), to acid-soluble ribonucleotides in a total reaction volume of 50 μl in 20 minutes at 37°C in 1X RNase H, E. coli Reaction Buffer with 10 nmol radiolabeled poly(rA) and 12.5 μg poly(dT). This product is distributed for laboratory research only. Caution: Not for diagnostic use. |
Supporting Protocol