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RecBCD (Exonuclease V), E. coli

CAT.NO UNIT
E093 100 U (100 μl)
Description RecBCD (Exonuclease V) is a Mg2 and ATP-dependent enzyme possessing exonuclease activity on linear double stranded (ds) DNA. The exonuclease digests DNA from both the 5’ and 3’ ends to produce oligonucleotides. RecBCD (Exonuclease V) does not degrade circular dsDNA such as plasmid DNA.
SKU E093
Unit quantity 100 U (100 μl)
Applications
  • Degradation of linear dsDNA while preserving circular plasmid dsDNA.
  • Clean up of chromosomal DNA from plasmid DNA after extraction.
Concentration 1000 U/ml
Format RecBCD enzyme supplied with 10X RecBCD Reaction Buffer. ATP is not provided. Incubate at 37°C.
Unit Definition One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble deoxyribonucleotide from double-stranded DNA in 30 minutes at 37°C in a total reaction volume of 50 µl.
Storage Buffer 50 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, 0.1% Triton X-100, pH 7.5 @ 25°C.
Note This product is distributed for laboratory research only.

Caution: Not for diagnostic use.

Supporting Protocol