페이지 선택

HOME ​ Exonuclease III, E. coli

Exonuclease III, E. coli

CAT.NO UNIT
E016 10 000 U (100 μl)
Description Exonuclease III, E.coli digests duplex DNA in the 3′→5′ direction from nicked DNA, blunt end DNA, 3′-recessed ends, or 3′-overhangs of less than four bases, and yields nucleoside 5′-phosphates. The DNA degradation proceeds at a uniform rate and produces stretches of ssDNA on the opposite strand. Under defined reaction conditions, the reaction can yield predictable and reproducible digestion results. Conditions such as temperature, ionic strength, template DNA sequence and the Exonuclease III to DNA ratio need to be optimized to suit specific applications to achieve the desired excision rate. Exonuclease III, E.coli is also capable of degrading DNA from 3′-phosphate ends due to intrinsic 3′-phosphatase activity. The enzyme also has apurinic DNA endonuclease activity as well as RNase H activity.
SKU E016
Unit quantity 10 000 U (100 μl)
Applications
  • Generation of intermediates for site-directed mutagenesis
  • Preparation of strand-specific radio labelled probes
  • Preparation of single-stranded DNA
  • Preparation of single-stranded templates for dideoxy-sequencing of DNA
  • Creation of unidirectional deletions in DNA fragments
  • Note: This product is not suitable for NGS or second strand cDNA synthesis applications.
Concentration 100 U/μl
Format Enzyme supplied with 10X Reaction Buffer
Storage Condition Store all components at -20°C.
Storage Buffer 20 mM Tris-HCl (pH 7.5), 100 mM KCl, 1 mM DTT, and 50% (v/v) Glycerol.
Note One unit is defined as the amount of Exonuclease III, E. coli that is required to catalyze the release of 1 nmol of acid soluble nucleotides from double stranded DNA in 30 minutes at 37°C in 1X Exonuclease III Reaction Buffer.
This product is distributed for laboratory research only. Caution: Not for diagnostic use.

Supporting Protocol