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DNase I (RNAse-Free)
DNase I (RNAse-Free)
| CAT.NO | UNIT |
|---|---|
| E091 | 200 U (100 μl) |
| Description | Deoxyribonuclease I (DNase I; EC 3.1.21.1) is a non-specific endonuclease that catalyzes the cleavage of phosphodiester bonds in single/double-stranded DNA, chromatin, and RNA-DNA hybrids. DNase I cleaves DNA to release di-and/or oligonucleotide (5’-phosphorylated and 3’-hydroxylated) end-products. This product can be used for RNA applications. |
|---|---|
| SKU | E091 |
| Unit quantity | 200 U (100 μl) |
| Applications | • Removes DNA from protein preparations and RNA samples • Mediates nick translation • Generates random fragments for dideoxy sequencing • Degrade template DNA following in vitro transcription • Mediate DNase I foot-printing |
| Concentration | 2 U/μl |
| Note | One unit is defined as the amount of DNase I that catalyzes the degradation of 1 μg of DNA in 10 minutes at 37 °C into tetranucleotides or smaller fragments.
This product does not contain any RNase inhibitors, so it is recommended to be used in combination with RNaseOFF Ribonuclease Inhibitor (Cat. G138). This product should not be used in digestions longer than 15 minutes or at temperatures higher than 37°C, or the residual RNase activity will begin to degrade the RNA. This product is distributed for laboratory research only Caution: Not for diagnostic use. |
Supporting Protocol
