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E. coli DNA Polymerase I
E. coli DNA Polymerase I
| CAT.NO | UNIT |
|---|---|
| G469 | 500 U (100 μl) |
| Description | E.coli DNA Polymerase I is a DNA-dependent DNA polymerase with intrinsic 3’→5’ and 5’→3’ exonuclease activities. Nick translation is facilitated by this enzyme due to the removal of nucleotides ahead of the growing chain via 5´→3´ exonuclease activity. |
|---|---|
| SKU | G469 |
| Unit quantity | 500 U (100 μl) |
| Applications |
Note: This product is not suitable for NGS or second strand cDNA synthesis applications. |
| Concentration | 5 U/μl |
| Format | Enzyme supplied with 10X Reaction Buffer |
| Storage Condition | Store all components at -20°C. |
| Storage Buffer | 25 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol. |
| Note | One unit is defined as the amount of E. coli DNA Polymerase I that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer. This product is distributed for laboratory research only. Caution: Not for diagnostic use. |
