DNA Polymerase I Large (Klenow) Fragment | ITSBio

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DNA Polymerase I Large (Klenow) Fragment

CAT.NO	UNIT
E013	500 U (100 μl)

Description	DNA Polymerase I Large (Klenow) Fragment is the large fragment of E. coli DNA Polymerase I (abm Cat. No. G469). The Klenow Fragment retains the DNA-dependent DNA polymerase activity of the E. coli DNA Polymerase I but lacks the 5’→3’ exonuclease activity. With its inherent 3’→5’ exonuclease activity, Klenow possesses the polymerization fidelity of the holoenzyme without degrading 5'-termini.
SKU	E013
Unit quantity	500 U (100 μl)
Applications	DNA blunting by filling-in 5'-overhangs with unlabeled or labeled dNTPs cDNA second-strand synthesis Generate single-stranded DNA probes using random primers Site-directed DNA mutagenesis using synthetic oligonucleotides Dideoxy DNA sequencing of single- or double-stranded DNA templates 3’→5’ exonuclease activity can blunt a 3’-overhang
Concentration	5 U/μl
Format	Enzyme supplied with 10X Reaction Buffer
Storage Condition	Store all components at -20°C.
Storage Buffer	25 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol.
Note	One unit is defined as the amount of DNA Polymerase I Large (Klenow) Fragment that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer. This product is distributed for laboratory research only. Caution: Not for diagnostic use.

Supporting Protocol

Large Klenow Fragment Data Sheet