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DNA dA-Tailing Kit
DNA dA-Tailing Kit
| CAT.NO | UNIT |
|---|---|
| E009 | 25 Reactions |
| Description | The DNA dA-Tailing Kit efficiently adds a non-template dAMP (dA) to the 3’ end of a blunt-ended DNA fragment. This incorporated 3’-dA prevents concatemer formation and prepares the DNA fragment for subsequent ligation of adaptors or cloning vectors that have complementary 3’-dT overhangs. The Klenow Fragment (3′→ 5′ Exo-) in this kit adds the 3’-dA via its DNA polymerase activity. This enzyme lacks 5’→3’ exonuclease activity and has mutations that effectively abolish the inherent 3’→5’ exonuclease activity, thus preventing degradation of this 3’-dA. This kit has been optimized to prepare compatible overhangs for the next step of DNA sample preparation for next generation sequencing. The Klenow Fragment (3′→ 5′ Exo-) enzyme is also available as a stand-alone enzyme (Cat. No. E038). |
|---|---|
| SKU | E009 |
| Unit quantity | 25 Reactions |
| Applications |
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| Storage Condition | Store all components at -20°C in a non-frost-free freezer. All components are stable for 1 year from the date of shipping when stored and handled properly. |
| Storage Buffer | 25 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, and 50% (v/v) Glycerol. |
| Note | One unit is defined as the amount of Klenow Fragment (3’→5’ Exo-) that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer. This product is distributed for laboratory research only. Caution: Not for diagnostic use. |
