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Pro Ligation-Free Cloning Kit (20 reactions)

CAT.NO UNIT
E086 20 Reactions
Description The Pro Ligation-Free Cloning Kit allows for the robust assembly of multiple DNA fragments in one step. Using a ligation-independent cloning method, as many as six DNA fragments are assembled seamlessly without concern for restriction enzyme site availability. abm’s Pro Ligation-Free cloning is a simple yet highly versatile method to rapidly clone highly complex, multiple component constructs.
SKU E086
Unit quantity 20 Reactions
Applications
  1. Rapid multi-fragment cloning and assembly of whole vector constructs.
  2. Clone in a promoter, gene, and tag, all in one step.
  3. Rapid assembly of CRISPR multiplex gRNA vectors in one step.

Sherman, S. L. . "Cofactor-Independent Pinacolase Directs Non-Diels-Alderase Biogenesis of the Brevianamides" : ().