Ligation-Free Cloning Kit

HOME Ligation-Free Cloning Kit

CAT.NO	UNIT
E002	100 Reactions

Description	The Ligation-Free Cloning kit is a versatile system to meet the demands of routine cloning projects. The progressive design has eliminated the requirement for compatible ends and unique restriction sites within the vector and inserts to be cloned. At the foundation of Ligation-Free Cloning technology is abm’s proprietary Ligation-Free Enzyme Mix which reliably facilitates the assembly of up to 3 DNA inserts (PCR-generated sequences) and a linearized vector by recognizing a 15 bp overlap region at both ends. This 15 bp overlap can be engineered by designing primers for amplification of your desired sequences to create inserts free from any redundant or unwanted base pairs. To eliminate the possibility of mutations associated with PCR amplification, scientists at abm have developed a DNA Polymerase with the highest proof-reading capability thus making this system the most reliable on the market.
SKU	E002
Unit quantity	100 Reactions
Applications	multiple fragment cloning and assembly. cloning of any insert into any location of a chosen vector. complete elimination of the dependence on availability of restriction sites, phosphatase treatment and ligation. inserts free from any redundant or unwanted base pairs. save over 50% on reagent costs in comparison to other products available on the market.

Supporting Protocol

MSDS
QC
Other

Can the reaction mix be incubated at 50C instead of on ice?

It is not recommend to incubate at 50C because the enzymes do not work efficiently at such a high temperature.

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