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XhoI
XhoI
| CAT.NO | UNIT |
|---|---|
| E077 | 10000 U (500ul) |
| SKU | E077 |
|---|---|
| Unit quantity | 10000 U (500ul) |
| Applications | Restriction enzyme digestion of double-stranded DNA |
| Components | XhoI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204) |
| Concentration | 20000 U/ml |
| Expression System | An E. coli strain that carries the cloned and modified XhoI gene from Xanthomonas holcicola (ATCC 13461). The XhoI enzyme recognizes and cuts the sequence C^TCGAG. |
| Inactivation Protocol | 65°C for 20 min |
| Reaction Definition | 1X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204). Incubate at 37°C. |
| Reaction Buffer | Buffer 1: 100%, Buffer 2: 25%, Buffer 3: 10%, Universal Buffer: 100% Click here to view the buffer compatibility chart for restriction enzymes. |
| Storage Condition | -20°C |
| Storage Buffer | 0.1 mM EDTA, 50% Glycerol, 1mM DTT, 10 mM Tris-HCl, 50 mM KCl, 200 µg/ml BSA, pH 7.4 @ 25°C. |
| Note | Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Impaired |
