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SmaI
SmaI
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| CAT.NO | UNIT |
|---|---|
| E074 | 10000 U (500 μl) |
| SKU | E074 |
|---|---|
| Unit quantity | 10000 U (500 μl) |
| Applications | Restriction enzyme digestion of double-stranded DNA. |
| Expression System | An E. coli strain that carries the cloned and modified SmaI gene from Serratia marcescens (ATCC 49779). The SmaI enzyme recognizes and cuts the sequence CCC^GGG. |
| Inactivation Protocol | 65°C for 20 min |
| Reaction Definition | 1X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204). Incubate at 37°C. |
| Reaction Buffer | Buffer 1: 100%, Buffer 2: 25%, Buffer 3: 10%, Universal Buffer: 100% Click here to view the buffer compatibility chart for restriction enzymes. |
| Storage Condition | -20°C |
| Storage Buffer | 50 % Glycerol, 1 mM DTT, 10 mM Tris-HCl, 300 mM NaCl, 500 µg/ml BSA, 0.1 mM EDTA, pH 7.4 @ 25°C. |
| Note | Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Blocked |
