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NotI
NotI
| CAT.NO | UNIT |
|---|---|
| E051 | 10000 U (500 µl) |
| SKU | E051 |
|---|---|
| Unit quantity | 10000 U (500 µl) |
| Applications | Restriction enzyme digestion of double-stranded DNA |
| Components | NotI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204) |
| Concentration | 20000 U/ml |
| Expression System | An E. coli strain that carries the modified NotI gene from Nocardia otitidis-caviarum (ATCC 14630). The NotI enzyme recognizes and cuts the sequence GC^GGCCGC. |
| Inactivation Protocol | 65°C for 20 min |
| Reaction Definition | 1X Universal Restriction Enzyme Reaction Buffer (Cat. No. E204). Incubate at 37°C |
| Reaction Buffer | Buffer 1: 25%, Buffer 2: 100%, Buffer 3: 25%, Universal Buffer: 100% Click here to view the buffer compatibility chart for restriction enzymes. |
| Storage Condition | -20°C |
| Storage Buffer | 10 mM Tris-HCl (pH 7.5), 250 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.15% Triton® X-100, and 50% (v/v) Glycerol |
| Note | Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Blocked by Some Combinations of Overlapping |
