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NdeI
NdeI
| CAT.NO | UNIT |
|---|---|
| E058 | 10000 U (500 µl) |
| SKU | E058 |
|---|---|
| Unit quantity | 10000 U (500 µl) |
| Applications | Restriction enzyme digestion of double-stranded DNA |
| Components | NdeI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204) |
| Concentration | 20000 U/ml |
| Expression System | An E. coli strain that carries the NdeI gene from Neisseria denitrificans (NRCC 31009). The NdeI enzyme recognizes and cuts the sequence CA^TATG. |
| Inactivation Protocol | No |
| Reaction Definition | 1X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204). Incubate at 37°C |
| Reaction Buffer | Buffer 1: 75%, Buffer 2: 100%, Buffer 3: 100%, Universal Buffer: 100%. Click here to view the buffer compatibility chart for restriction enzymes. |
| Storage Condition | -20°C |
| Storage Buffer | 10mM Tris pH (7.5), 100mM NaCl, 1mM DTT, 0.1mM EDTA, and 50% (v/v) Glycerol. |
| Note | Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Not Sensitive DNA isolated using standard miniprep methods may be cut with lower activity. |
