HOME 
KpnI
KpnI
| CAT.NO | UNIT |
|---|---|
| E054 | 20000 U (1 ml) |
| SKU | E054 |
|---|---|
| Unit quantity | 20000 U (1 ml) |
| Applications | Restriction enzyme digestion of double-stranded DNA |
| Components | KpnI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204) |
| Concentration | 20000 U/ml |
| Expression System | An E. coli strain that carries the KpnI gene from Klebsiella pneumoniae OK8 (ATCC 49790). The KpnI enzyme recognizes and cuts the sequence GGTAC^C. |
| Inactivation Protocol | No |
| Reaction Definition | 1X Universal Restriction Enzyme Reaction Buffer (Cat. No. E204). Incubate at 37°C |
| Reaction Buffer | Buffer 1: 100%, Buffer 2: 25%, Buffer 3: 10%, Universal Buffer: 100% Click here to view the buffer compatibility chart for restriction enzymes. |
| Storage Condition | -20°C |
| Storage Buffer | 10 mM Tris-HCl (pH 7.5), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, and 50% (v/v) Glycerol |
| Note | Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Blocked by Some Combinations of Overlapping |
