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10X Restriction Enzyme Reaction Buffer 3
10X Restriction Enzyme Reaction Buffer 3
| CAT.NO | UNIT |
|---|---|
| E070 | 5000 U (500ul) |
| SKU | E070 |
|---|---|
| Unit quantity | 5000 U (500ul) |
| Applications | Restriction enzyme digestion of double-stranded DNA |
| Components | HaeIII enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204). |
| Concentration | 10000 U/ml |
| Expression System | An E. coli strain that carries the cloned and modified HaeIII gene from Haemophilus aegypticus (ATCC 11116). The HaeIII enzyme recognizes and cuts the sequence GG^CC. |
| Inactivation Protocol | 80°C for 20 min |
| Reaction Definition | 1X Universal Restriction Enzyme Buffer (Cat.No. E204). Incubate at 37°C. |
| Reaction Buffer | Buffer 1: 50%, Buffer 2: 25%, Buffer 3: 10%, Universal Buffer: 100% Click here to view the buffer compatibility chart for restriction enzymes. |
| Storage Condition | -20°C |
| Storage Buffer | 0.1 mM EDTA, 50% Glycerol, 1 mM DTT, 10 mM Tris-HCl, 50 mM KCl, 200 µg/ml BSA, pH 7.4 @ 25°C. |
| Note | Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Not Sensitive |
