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HaeII
HaeII
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| CAT.NO | UNIT |
|---|---|
| E060 | 10000 U (500 µl) |
| SKU | E060 |
|---|---|
| Unit quantity | 10000 U (500 µl) |
| Applications | Restriction enzyme digestion of double-stranded DNA |
| Components | HaeII enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204) |
| Concentration | 20000 U/ml |
| Expression System | An E. coli strain that carries the HaeII gene from Haemophilus aegypticus (ATCC 11116). The HaeII enzyme recognizes and cuts the sequence RGCGC^Y. |
| Inactivation Protocol | 80°C for 20 min |
| Reaction Definition | 1X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204). Incubate at 37°C |
| Reaction Buffer | Buffer 1: 25%, Buffer 2: 100%, Buffer 3: 10%, Universal Buffer: 100%. Click here to view the buffer compatibility chart for restriction enzymes. |
| Storage Condition | -20°C |
| Storage Buffer | 10 mM Tris-HCl (pH 7.5), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, and 50% (v/v) Glycerol. |
| Note | Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Blocked |
