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BbsI

 

CAT.NO UNIT
E076 1500 U (75 μl)
SKU E076
Unit quantity 1500 U (75 μl)
Applications Restriction enzyme digestion of double-stranded DNA
Components BbsI enzyme supplied with 10X Restriction Enzyme Reaction Buffer 2 (Cat.No. E202)
Concentration 20000 U/ml
Expression System An E. coli strain that carries the cloned and modified BbsI gene from Bacillus laterosporus (C. Story). The BbsI enzyme recognizes and cuts the sequence GAAGAC(N2)^.
Inactivation Protocol 65°C for 20 min
Reaction Definition 1X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204). Incubate at 37°C.
Reaction Buffer Buffer 1: 10%, Buffer 2: 10%, Buffer 3: 10%, Universal Buffer: 100%
Click here to view the buffer compatibility chart for restriction enzymes.
Storage Condition -80°C
Note Methylation Sensitivity
dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Not Sensitive

Supporting Protocol

MSDS
QC

Other