BbsI
CAT.NO | UNIT |
---|---|
E076 | 1500 U (75 μl) |
SKU | E076 |
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Unit quantity | 1500 U (75 μl) |
Applications | Restriction enzyme digestion of double-stranded DNA |
Components | BbsI enzyme supplied with 10X Restriction Enzyme Reaction Buffer 2 (Cat.No. E202) |
Concentration | 20000 U/ml |
Expression System | An E. coli strain that carries the cloned and modified BbsI gene from Bacillus laterosporus (C. Story). The BbsI enzyme recognizes and cuts the sequence GAAGAC(N2)^. |
Inactivation Protocol | 65°C for 20 min |
Reaction Definition | 1X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204). Incubate at 37°C. |
Reaction Buffer | Buffer 1: 10%, Buffer 2: 10%, Buffer 3: 10%, Universal Buffer: 100% Click here to view the buffer compatibility chart for restriction enzymes. |
Storage Condition | -80°C |
Note | Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Not Sensitive |