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abm Molecular Biology and PCR RNA Visualization
RNA Visualization
RNA Mango
The most advanced RNA tracking, visualization and pull down technology.Technology for studying the diverse cellular roles of RNA has lagged behind the tools for studying DNA and proteins, but innovative researchers are working to change that! One such researcher is Dr. Peter Unrau of Simon Fraser University. He and his team have created RNA Mango, a novel technology with a number of useful applications.
Key Features
RNA Mango technology is based on the specific binding of the RNA Mango Aptamer and a Thizole Orange (TO) bi-functional dye. The main features of this technology is the tight binding between the dye and aptamer (KD ≈ 3nM) , and the strong ~1000X enhancement of the dye’s fluorescence when bound to the Mango aptamer (Fluorescent enhancement FE=1,100).
The TO dye has a number of other desirable properties including:
- Small size
- Lack of toxicity
- Plasma and nuclear membrane permeability
- Short intracellular half-life
- The accessibility of a broad wavelength range simply via substitutions and alterations to the TO structure
TO1-biotin is the standard variety of TO dye for RNA Mango experiments; other dye variants will also be available soon.
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Popular Products
Related Products
The system has two components: the RNA Mango aptamer, and the TO-1 dye. The dye only fluoresces when bound to the Mango aptamer.
For mRNA inset the tag into the 3’UTR. For structured non-coding RNA replace a stem-loop that is not essential to the RNA function.
Express RNA of interest (with Mango Tag) using a vector or CRISPR Knock-In. Soak cells in TO-1 Biotin dye to illuminate RNA of interest localization
Express RNA of interest (with Mango Tag) using a vector or CRISPR Knock-In. Lyse the cells, and recover RNA of interest with bound RNA or proteins using streptavidin breads
Laboratory Results
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| Yeast U1 Ribonucleoprotein (RNP) Complex Pulldown Purification Left: RNAs present in native extract in which U1M migrates as doublet. Right: U1M is a single band RNP present after mango-based purification using TO1 3PEG-Desthiobiotin Fluorophore |
RNA Mango in a Test Tube Mango dye binding to in vitro transcribed RNA Mango-tagged sgRNA. Stable binding and fluorescence even after leaving the tube for 1 month at room temperature. |
Products
| SERVICE | UNIT | CAT.NO. |
|---|---|---|
| TO1-3PEG-Biotin Fluorophore | 0.5 mg/ml (500 µl) | G955 |
| TO1-3PEG-Desthiobiotin Fluorophore | 0.5 mg/ml (500 µl) | G956 |
| TO3-3PEG-Biotin Fluorophore | 0.5 mg/ml (500 µl) | G959 |
| YO3-3PEG-Biotin Fluorophore | 0.5 mg/ml (500 µl) | G957 |
Top Publications
| 01 |
Ribonucleoprotein purification and characterization using RNA Mango. Panchapakesan SSS, Ferguson ML, Hayden EJ, Chen X, Hoskins AA, Unrau PJ. et al. DOI: 10.1261/rna.062166.117. PubMed: 28747322. PubMed Central PMCID: PMC5602116.
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| 02 |
Structural basis for high-affinity fluorophore binding and activation by RNA Mango. Trachman RJ 3rd, Demeshkina NA, Lau MWL, Panchapakesan SSS, Jeng SCY, Unrau PJ, Ferré-D’Amaré AR. et al. DOI: 10.1038/nchembio.2392. Epub 2017 May 29. PubMed : 28553947.PubMed Central PMCID: PMC5550021.
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| 03 |
Fluorophore ligand binding and complex stabilization of the RNA Mango and RNA Spinach aptamers. Jeng SC, Chan HH, Booy EP, McKenna SA, Unrau PJ. et al |
