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Mouse Monoclonal Antibody Production

Key requirements for successful monoclonal antibody generation include immunization strategies, antigen design, and screening technologies. abm‘s novel immunization procedures and careful mouse strain selection allows for production of monoclonal antibodies which are of the utmost affinity and specificity. This is because we take great care in designing the antigens for antibody production, especially with respect to synthesizing peptides, to generate a robust antibody that is able to recognize conformational changes of the native protein under the optimum number of conditions.

Mouse monoclonal antibody production is divided into different phases, with a non-refundable $1500.00 to cover the material cost to initiate the project. The balance will be due upon service completion. Our price includes antigen protein production. All you need to do is give us the gene accession number and we will take care of everything else. This unique service is only available from

“I am a fan of abm: great product quality, great price, great rewards and great experience.”

Dr. Vincent Emond, Centre de recherche du CHU de Québec CHUL

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Service Details

Core Services

SERVICE DESCRIPTION CAT. NO. UNIT
Complete Package with peptide production
  1. Peptide antigen production
  2. Immunization (5 mice/antigen) and Sera Testing by ELISA
  3. Fusion and Colony Screening
  4. Subcloning, Colony Expansion and Freezing
  5. Antibody Purification from Ascites by Protein A/G (2 clones, 3mg each)
 C080 1 Service
Complete Package with protein production
  1. Protein antigen production
  2. Immunization (5 mice/antigen) and Sera Testing by ELISA
  3. Fusion and Colony Screening
  4. Subcloning, Colony Expansion and Freezing
  5. Antibody Purification from Ascites by Protein A/G (2 clones, 3mg each)
 C487 1 Service
Standard Package without antigen production
  1. Immunization (5 mice/antigen) and Sera Testing by ELISA
  2. Fusion and Colony Screening
  3. Subcloning, Colony Expansion and Freezing
  4. Antibody Purification from Ascites by Protein A/G (2 clones, 3mg each)
 C087 1 Service
 

Add-On Services

SERVICE DESCRIPTION CAT. NO. UNIT
Additional Clone(s) Steps 4 and 5 C081 1 Clone
Production from 1-25 Mice C082 1 Mouse
Production from 26-50 Mice C083 1 Mouse
Production from 51-100 Mice C084 1 Mouse
Ascites Production Production from 101-150 Mice C085 1 Mouse
Production from 151-200 Mice C086 1 Mouse
Production from 200+ Mice Inquire 1 Mouse

Amount of antigen to submit for Standard Package Service:
For peptide antigens, please submit at least 2mg. For protein antigens, please submit at least 5mg with concentrations of 0.5-1.0mg/ml.

Additional Info

Note:

  • Customers can provide a peptide antigen or a ready-to-inject antigen for monoclonal antibody production. When using a peptide antigen supplied by the customer, an additional charge of $200.00 is applied for KLH conjugation.
  • Customers have the option to start or stop the project from any stages of the mouse monoclonal antibody generation service. Customers will be responsible for the material and labour cost incurred thus far should early termination be requested.

 

Documents

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FAQs

To produce an antibody that has specificity and affinity at acceptable levels, we require a minimum of 20 amino acids.

Yes, however to guarantee the antibody is effective against the native protein purified native protein must be used for immunization. Recombinant protein or peptide extracts can also be used to immunize, but we cannot guarantee these will be effective against the native protein due to folding and other conformational issues. We only guarantee that the immunized protein (native or recombinant) or peptide will be recognized by the produced antibody.

Our process mainly results in IgG2a and IgG2b antibodies.

For peptide antigens we require at least 2mg, while if it is a protein 5mg with concentrations of 0.5-1.0mg/ml are acceptable.

Please inquire with quotes@abmgood.com

Please provide: 1) The peptide antigen design 2) Mouse monoclonal or Polyclonal Antibody Production? 3) Are you looking for the standard service or complete service? Please email this information to quotes@abmgood.com

Citations

01 Ko, FC et al. “PKA-induced dimerization of the RhoGAP DLC1 promotes its inhibition of tumorigenesis and metastasis.” Nat Commun 4:1618 (2013). PubMed: 23511482