Several unique features of HSV-1 make vectors derived from this virus very appealing for preventive or therapeutic gene transfer. These include:

• Delivery of large transgenes to the nucleus of mammalian cells.
• Non-integrating, but can replicate separately from the host cell genome.
• The ability to establish life-long, non-toxic, latent infections in neurons; can establish stable transgene expression.
• Based on the HSV amplicon packaging method, which yields higher titers and has lower toxicity.
• All custom HSVs come with a GFP reporter for easy tracking of infection into target cells.

By pairing this technology with our expertise in virus packaging, abm offers custom HSV-1 Virus subcloning and virus production, allowing high-level gene expression in host cells.

Custom Baculovirus Plasmid Cloning

Custom Cloning Service

Custom HSV Production

HSV Controls

1. ***Cloning of genes over 3kb are subject to an additional charge of $100.00/kb (C100). Please contact technical@abmgood.com for more information on available cloning vectors and restriction enzyme sites to qualify for the compatible insert service.
2. C098: The above services must be ordered with a Custom HSV Production service, either C158 or C159, and a Custom HSV Cloning service, either C173 or C174.
3. For customer-supplied vectors, the customer must indicate prior to the service if any reporter expression will be expected or not. The expected reporter expression, e.g. GFP, should not require induction of expression. Due to differences in excitation/emission wavelengths for different fluorophores, we may not be able to provide infection images for fluorescent reporter expression other than standard GFP/RFP/mCherry.
4. The above services must be ordered with a Custom HSV Production service, either C158 or C159.

Herpes simplex virus (HSV) is a neurotropic herpes virus that can establish lifelong infections in its human host through latent maintenance in the ganglia of sensory neurons. Because of their wide host range, efficient infection, long-term persistence, capacity to accommodate large amounts of foreign DNA, and ability to deliver genes to post-mitotic cell types, HSV has showed considerable promise to establish long-term gene expression in most cells, particularly in neurons where other systems have low efficiency. Helper-free and amplicon HSV vectors have deletions in one or more essential viral genes and these can be used to express transgenes by replacing other non-essential viral genes.

Custom Lentivirus

Custom AAV

Recombinant Adenovirus