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Custom Retrovirus Services
abm’s custom retroviruses are a highly-efficient method to achieve stable gene expression in a broad range of dividing mammalian cells. Simply send us your gene, or purchase an ORF vector from our extensive library of human, mouse, or rat genes, and we will do the rest! Our packaged retroviruses are available with your choice of no tag, HA tag, or GFP reporter.
| KEY FEATURES OF abm’s CUSTOM RETROVIRUS SERVICE | |
|---|---|
| Promoter | CMV promoter for strong gene expression in most cellular systems |
| Tags Available | No tag HA tag for detection or protein purification GFP reporter |
| Retovirus TIters | Up to 106 IU/ml |
| Safety | Replication-incompetent retrovirus with minimal relation to the wild-type Moloney Murine Leukemia Virus (MoMuLV) |
“abm has always been very helpful with technical assistance in any of our questions and concerns. I also consider the price for the services provided as very competitive, as other companies charge a multiple of abm‘s price.”
Dr. Cameron Moshfegh, ETH Zurich
Additional Resources:
Service Details
Custom Retroviral Vectors
| SERVICE NAME | DESCRIPTION | UNIT | CAT. NO. |
|---|---|---|---|
| Custom Retroviral Vector using ORF Library with No Tag | Any human, mouse, or rat gene from our extensive ORF library, available in a retroviral vector with no tag. Corresponding ORF vector must be ordered with this service. Maximum insert size that can be packaged is 4.5 kb. |
1.0 μg | C362 |
| Custom Retroviral Vector using ORF Library with HA Tag | Any human, mouse, or rat gene from our extensive ORF library, available in a retroviral vector with a C-term HA tag. Corresponding ORF vector must be ordered with this service. Maximum insert size that can be packaged is 4.5 kb. |
1.0 μg | C363 |
| Custom Retroviral Vector using ORF Library with GFP Reporter | Any human, mouse, or rat gene from our extensive ORF library, available in a retroviral vector with a GFP reporter. Corresponding ORF vector must be ordered with this service. Maximum insert size that can be packaged is 3.2 kb. |
1.0 μg | C364 |
| Custom Retroviral Vector | Custom retrovirus plasmid subcloning. Provide your DNA and we’ll subclone it into a retroviral vector. | 1 Service | C057 |
| Gene Synthesis | For any sequence up to 4.5 kb in size. Genes are fully-sequenced. Must be ordered with Custom Retroviral Vector (C362, C363 or C364). * Minimum charge of $148.50 |
1.0 μg | C098 |
Additional cloning services are available.
Custom Retroviruses
| SERVICE NAME | QUANT. DNA PROVIDED | CAT. NO. | QUANT | |
|---|---|---|---|---|
| Custom Retrovirus | Enough DNA Provided | ≥50 μg | C058 | 10 ml of 105 IU/ml |
| ≥50 μg | C059 | 10 ml of 106 IU/ml | ||
| DNA Amplification Required | <50 μg | C064 | 10 ml of 105 IU/ml | |
| <50 μg | C065 | 10 ml of 106 IU/ml | ||
Please Note: For customer-supplied vectors, the customer must indicate prior to the service if any reporter expression will be expected or not. The expected reporter expression, e.g. GFP, should not require induction of expression. Due to differences in excitation/emission wavelengths for different fluorophores, we may not be able to provide infection images for fluorescent reporter expression other than standard GFP/RFP/mCherry.
Retrovirus Controls
| PRODUCT | UNIT | CAT. NO. |
|---|---|---|
| pRetroG-CMV-blank Vector | 1.0 μg | RV001 |
| pRetroG-CMV-GFP-HA Vector | 1.0 μg | RV002 |
| pRetroG-CMV-GFP-stop Vector | 1.0 μg | RV003 |
| pRetroG-CMV-GFP-IRES-GFP Vector | 1.0 μg | RV004 |
| pRetroG-CMV-RFP-stop Vector | 1.0 μg | RV005 |
| Retro-CMV Blank Retrovirus | 1 ml of 106 IU/ml | RVP001 |
| Retro-CMV-GFP-HA Retrovirus | 1 ml of 106 IU/ml | RVP002 |
| Retro-CMV-GFP Retrovirus | 1 ml of 106 IU/ml | RVP003 |
| Retro-CMV-GFP-IRES-GFP Retrovirus | 1 ml of 106 IU/ml | RVP004 |
| Retro-CMV-RFP Retrovirus | 1 ml of 106 IU/ml | RVP005 |
Retrovirus Vectors (click for details):
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Additional Info
Related Products
FAQs
How long does it take to produce virus?
This will be dependent on a number of factors, including the plasmid you would like to package. Please send all inquiries to technical@abmgood.com for a prompt response on the estimated lead time.
What the concentration of transfer vector should I submit?
The volume of DNA needed to produce recombinant virus has been listed alongside each titre available. (see above)
How do you determine titer?
Via RT-PCR
What is the packaging capacity for Retrovirus?
The maximum insert size is <8 kb between the 5'LTR to 3'LTR.
What is the minimum amount of DNA needed for subcloning into a retroviral vector (C057)?
The minimum amount of DNA we require is 10μg for subcloning into a retroviral vector.
Citations
| 01 | Thomas, A. M. et al. “Polysaccharide-modified scaffolds for controlled lentivirus delivery in vitro and after spinal cord injury.” J Control Release. 3:421-429 (2013). DOI: 10.1016/j.jconrel.2013.06.013 PubMed: 23791981 |
