Though the BAC is much larger than regular plasmid, we can still offer this service as long as the BAC is under 15kb. For BAC service, a special quotation has to be offered, depending on the nature of a particular plasmid. The digestion will cost another $100~200/100ug depending which enzymes to be used as some enzymes are more expensive than others.

Please send in at least 1 μg of plasmid DNA or your transformed bacteria. Information for the host bacteria is required if you are sending transformed bacteria with your plasmids. Also, please provide the antibiotic selection marker if available.

Number of plasmid. Amount of DNA for each plasmid. Plasmid map to check for transformation strain compatibility. Antibiotic for proper plasmid selection. Insert name so it can be confirmed to not be toxic or pathogenic. High or low copy plasmid.

Typically, we request to receive the plasmids in liquid format as purified DNA, since this is most stable. However, bacterial stocks would also be suitable, provided that they are shipped to us within 2-3 days of preparation to ensure that the bacteria retain the plasmid.