Every gene has a 5’ UTR and 3’ UTR (Untranslated Region) upstream and downstream, respectively, of the coding region. While 3’ UTRs are the major focus of regulatory research, 5’ UTRs have also been found to mediate translational regulation via elements such as upstream start codons (uAUGs), upstream open reading frames (uORFs), secondary structures, RNA binding protein (RBP) binding motifs, and miRNA binding.

abm’s 5’ UTR Reporter Vectors consist of the 5’ UTR of the gene of your choice preceding a GFP or Luciferase reporter, driven by a β-actin promoter. These vectors are ideal for studying the regulation of protein expression by 5’ UTR elements. The 5’ UTR reporter constructs can be combined with our Site-Directed Mutagenesis Service (Cat. No. C047) in order to define RBP or miRNA binding sites, or investigate the effects of uAUGs or uORFs on gene expression.

All of abm’s UTR reporter vectors are available as ready-to-use DNA plasmids, or they can be packaged into lentiviral particles. Our UTR reporter vectors can be directly transfected into target cells for transient expression, while lentiviral particles allow difficult-to-transfect cells, such as primary cells, to be efficiently transduced for stable, long-term expression.

Custom Cloning Services

Site Directed Mutagenesis service

Lentivirus Packaging Services