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abm Research CRO Custom Services Custom Promoter-Reporter Vector
Custom Promoter-Reporter Vector
We offer nearly 18,000 human and 17,500 mouse promoters, available in lentiviral vectors with luciferase or GFP reporters. These vectors can be used directly for plasmid transfection or for packaging into lentivirus to generate stable promoter-reporter cell lines for downstream cell assays.
Use abm’s Promoter-Reporter constructs to:
- examine the mechanisms that control gene expression or repression
- characterize transcription factor binding
- discover the effect of different promoter variants using our Site-Directed Mutagenesis Service (Cat. No. C047)
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| pLenti-Promoterless-GFP Vector | pLenti-Promoterless-Luc Vector |
Our promoter-reporter constructs consist of approximately -1500 bp upstream and +100 bp downstream of the transcription start site, driving the expression of a luciferase or GFP reporter in a lentiviral vector. This can be used directly for transfection or be packaged into virus for infection and Stable Cell Line Generation.
“Of all the companies I have reached out to, abm has been the best in terms of explaining the technology and how it fits my application.”
Tim Erickson, Ingenovax, Custom Cloning Service
Additional Resources:
Service Details
| PRODUCT | REPORTER | AMOUNT | CAT. NO. |
|---|---|---|---|
| Custom Promoter-Reporter Lentiviral Vector (GFP) | EGFP | 1.0 µg | C450 |
| Custom Promoter-Reporter Lentiviral Vector (Luc) | Firefly Luciferase | 1.0 µg | C449 |
| CONTROLS | REPORTER | AMOUNT | CAT. NO. |
|---|---|---|---|
| Blank Promoter-Reporter Lentiviral Vector (Luc) | Firefly Luciferase | 1.0 µg | LV653 |
| Blank Promoter-Reporter Lentiviral Vector (GFP) | EGFP | 1.0 µg | LV654 |
| CMV Promoter-Reporter Lentiviral Vector (Luc) | Firefly Luciferase | 1.0 µg | LV655 |
| CMV Promoter-Reporter Lentiviral Vector (GFP) | EGFP | 1.0 µg | LV656 |
| ADD-ONS | AMOUNT | CAT. NO. |
|---|---|---|
| Site-Directed Mutagenesis Service | Single Clone | C047 |
| Plasmid Amplification (10-20 µg) | 10‑20 µg | C309 |
| Plasmid Amplification (80-100 µg) | 80‑100 µg | C310 |
| Bacterial Glycerol Stock | 1 Clone | C139 |
| Custom Lentivirus Packaging Service | 106‑1010 IU/ml | Click here |
Need to study a larger promoter or multiple variations of a promoter? Combine this with our Custom Cloning Services or Site-Directed Mutagenesis service!
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Citations
| 01 | Xu, Y et al. “Bile acid and cigarette smoke enhance the aggressive phenotype of esophageal adenocarcinoma cells by downregulation of the mitochondrial uncoupling protein-2.” Oncotarget 8.60:101057–101071. (2017). DOI: 10.18632/oncotarget.22380 Application: Lentiviral transfection. |
