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SpeedySeq DNA Sequencing

With abm’s express DNA Sanger sequencing service, you can download your sequence data within a week of sample submission.

Select your free primers from our list of available universal primers, use our oligonucleotide synthesis service for unique primer design or send your own primers along with your samples.

We can solve many sequencing related problems, such as multiple hairpin loops and uneven base distributions. Our streamlined technology platforms and proprietary reagents ensure highly accurate performance, and our dedicated and highly experienced team is ready to assist with your special requirements.

 

“I am a fan of abm: great product quality, great price, great rewards and great experience.”

Dr. Vincent Emond, Centre de recherche du CHU de Québec CHUL

Additional Resources:

Service Details

 

SERVICE NAME UNIT CAT. NO.
SpeedySeq DNA Sequencing 1 Reaction C199
SpeedySeq DNA Sequencing with Custom Primer Synthesis 1 Reaction C197
PCR Cleanup for Sanger Sequencing 1 Sample C319

 

Guarantee: abm will repeat any poor quality sequencing reactions one time if requested within 10 business days of sequencing results becoming available for download. After this time frame, all repeat sequencing requests will be charged as new sequencing reactions requiring new sample submission.

Additional Info

  1. Download and fill out an order request form. Please include all of the required information such as your template names, concentrations, estimated sizes, and corresponding primer names, plus any special notes (high/low GC, multiple hairpins etc.).

    To qualify for SpeedySeq DNA Sequencing (C199) service, if your chosen primers are not on the list of Free Sequencing Primers, you will need to send us your primers along with your samples. Alternatively, if a primer is listed on the abm Free Sequencing Primers list (see documents section below), these will be provided as part of the service at no additional cost. When choosing our free sequencing primer(s), please use the primer name exactly as shown in the list, and leave the primer concentration cell blank.

    SpeedySeq DNA Sequencing with Custom Primer Synthesis (C197) service includes the custom synthesis of one sequencing primer of 20-30 bases in length to be used for sequencing your DNA sample. Please mention your desired primer sequence when submitting the order request form.

    Please provide your billing information & e-mail address. Your completed order request form can be attached and emailed to technical@abmgood.com.

  2. Once you have received an order confirmation email, please send your samples (plus primers where applicable) to us at:

    Attn: Order # xxxxxxxxx
    Applied Biological Materials Inc.
    1-3671 Viking Way
    Richmond, BC
    V6V 2J5 CANADA

    TEMPLATE TYPE DNA CONCENTRATION DNA VOLUME PRIMER CONCENTRATION PRIMER VOLUME
    Plasmid 100 ng/μl 10 μl 5 μM 10 μl
    PCR (100-500 bp) 5-10 ng/μl 10 μl 5 μM 10 μl
    PCR (500 bp-1 kbp) 10-20 ng/μl 10 μl 5 μM 10 μl
    PCR (>1.0 kbp) 20-40 ng/μl 10 μl 5 μM 10 μl
    Cosmid 0.5-1 μg/μl 20 μl 5 μM 10 μl
    Fosmid 0.5-1 μg/μl 20 μl 5 μM 10 μl
    Cosmid 0.5-1 μg/μl 20 μl 5 μM 10 μl
    BAC 0.5-1 μg/μl 20 μl 5 μM 10 μl
    Genomic DNA 2-3 μg/μl 20 μl 5 μM 10 μl
    *The volumes indicated above are for each sequencing reaction. For example, if submitting 4 plasmids, each to be sequenced using the same set of forward and reverse primers, please submit a total of 20 μl per plasmid, 40 μl of forward primer and 40 μl of reverse primer.

Documents

Order Request form

Free Sequencing Primers List

 

FAQs

Yes, we can certainly look into this for you. Please email technical@abmgood.com for further information.

Citations

Coming Soon