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Custom Cloning Service

A one-stop source for research solutions, abm offers gene synthesis, cloning, and virus packaging services aimed to reduce the time, effort and money spent by researchers.

abm‘s top-notch custom services include, but are not limited to:

  • subcloning
  • gene synthesis
  • codon optimization (upon request)
  • mutagenesis
  • expression vector customization:

    tissue-specific promoters

     

    ; fluorescent reporters; tags and lox sites; drug resistance markers

  • sequence verification of inserts in final vector; vector verification by restriction digest

Our experienced scientists will assemble your clone accurately, rapidly, economically, and ready to use for your experiment.

“Of all the companies I have reached out to, abm has been the best in terms of explaining the technology and how it fits my application.”

Tim Erickson, Ingenovax, Custom Cloning Service

Additional Resources:

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Service Details

SERVICE NAME DESCRIPTION UNIT CAT. NO.
Economy Gene Synthesis & Subcloning
  • For non-complicated sequences
  • Synthesize up to 950 bp
  • Complimentary subcloning into pUC57, EcoRV site
  • Sequence verification to confirm full length sequence is generated
  • Sequence must be approved for economy synthesis by abm
 1.0 μg C078
Gene Synthesis
  • For any sequence up to 4 kbp in size
  • Genes are fully sequenced
  • Must be ordered with Subcloning Service I (C096)
  • Minimum charge of USD$148.50
 1.0 μg C098
Subcloning Service I
  • ubclone any insert into an expression system of your choice. This service is intended for inserts with restriction sites that are compatible with the expression vector of choice.
  • End sequencing of the insert is performed with this service to confirm proper insertion.
  • For full sequencing, please refer to C197.
 1.0 μg C096
Subcloning Service II
  • Subclone any insert into an expression system of your choice. This service is intended for inserts that are not compatible with restriction sites of the expression vector of choice.
  • Full length DNA sequencing is performed for sequence verification.
  • Genes over 3 kbp are subject to an additional charge of $100.00/kbp (C100).
 1.0 μg C097
Site-directed Mutagenesis
  • Mutate up to 25 bp (consecutive) within a < 2 kbp fragment in any existing abm product or a provided vector.
  • Full length sequencing to confirm presence of mutation
  • Fragments over 2 kbp are subject to an additional charge of $100.00/kbp (C100).
 1.0 μg C047
Additional Charge for Extra 1 kbp
  • Additional charge for fragments over the stated size limit ($100.00/kbp)
  • Only applicable to C097, C047 and C128.
 N/A C100
Promoter Change Service
  • Change the promoter in one of abm’s vectors or a vector of your choice to any of the following: CMV, EF1α, PGK, UbC
 1.0 μg C126
Tag Addition/Removal Service (≤ 30 bp)
  • Addition/Removal of an N-terminal or C-terminal tag smaller than 30 bp
 1.0 μg C127
Tag Addition/Removal Service (> 30 bp) *
  • Addition/Removal of an N-terminal or C-terminal tag larger than 30 bp
  • Tags over 1 kbp are subject to an additional charge of $100.00/kbp (C100).
 1.0 μg C128
Reporter Addition/Removal Service
  • Addition, removal or switching of a reporter (e.g. GFP, RFP, Luc) in one of abm’s vectors or a vector of your choice.
 1.0 μg C129
Antibiotic Switching Service
  • Switching of a Puromycin resistance gene to Neomycin, Hygromycin or Blasticidin resistance in one of abm’s vectors or a vector of your choice.
 1.0 μg C140

ADD-ON-SERVICES 

SERVICE NAME DESCRIPTION UNIT CAT. NO.
DNA Sequencing
  • Verify the sequence and position of your gene of interest or sequence your PCR products.
 Single Reaction C199
Bacterial Agar Stab
  • Bacterial stab of the custom plasmid.
 1 clone C315
Plasmid Amplification (10-20 μg)
  • Amplification of the custom plasmid.
 10-20 μg C309
Plasmid Amplification (80-100 μg)
  • Amplification of the custom plasmid.
 80-100 μg C310

 

***Bulk amplification services available here

Additional Info

abm Vectors Available for Use in the Above Services
Cloning into pUC57 or pORF is free of charge; for use of other abm vector backbones the charge is $350 (Cat. No. C318). Here is a selected list of expression vectors. Cloning into retrovirus, HSV, baculovirus, and much more is also offered at abm.

LENTIVIRAL SYSTEM AAV SYSTEM ADENOVIRAL SYSTEM PROTEIN EXPRESSION SYSTEM
pLenti-III-HA pAAV-G-CMV-amp pShuttle (+) pPB-C-His
pLenti-EF1a-GFP-2A-Puro pAAV-G-EF1a-hGH-amp pShuttle (-) pPB-His-MBP
pLenti-CMV-GFP-2A-Puro pAAV-G-PGK-hGH-amp pShuttle-HA pPB-His GST
pLenti-CMV-RFP-2A-Puro pAAV-G-CAGGS-hGH-amp pShuttle-His pPB-N-His
pLenti-Bi-cistronic   pPromoter pPM-C-His
pLenti-Tri-cistronic   pPM-C-HA  
pLenti-III-EF1α   pPM-N-D-C-HA  
pLenti-III-PGK   pPM-N-D-C-His  
pLenti-III-UbC      
pLenti-Promoterless      
Additional lentiviral vectors available. Additional vectors available. Additional vectors available.

 

  • Viral packaging services available upon request.
  • We can also insert your gene in an inverse orientation, flanked by double lox sites, so that it will only flip for expression in cells that express Cre recombinase.
  • Try tissue-specific promoters for controlled gene expression.

Documents

Order Form for Custom Cloning Service

Viral Vector Brochure

Shipping Samples with abm

FAQs

Yes, we can certainly look into this for you. Please email technical@abmgood.com for further information.

Citations

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