Go beyond indel mutations! abm offers a custom CRISPR Gene Deletion and miRNA/lncRNA Knockout Service for unprecedented control of your CRISPR deletion.

CRISPR can be used to carry out large genomic deletions by utilizing dual sgRNAs, which target Cas9 cleavage upstream and downstream of the locus of interest. These CRISPR gene deletions benefit from the presence of a homology-directed repair (HDR) template, which allows for the precise repair of DNA via the HDR pathway. This ability is particularly useful for performing CRISPR lncRNA knockout or CRISPR miRNA knockout, as simple indel mutations do not facilitate effective knockout of miRNA or lncRNA. Order a gene deletion or miRNA/lncRNA knockout, and you’ll receive an edited cell line ready for use in downstream experiments.

Applications:

• Delete precise genomic sequences in your gene of interest
• Delete non-coding RNA sequences including microRNA (miRNA) and long non-coding RNA (lncRNA)

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CO₁ DNA Barcoding

• Confirms the cell line species of origin
• Utilizes sequence data the mitochondrial cytochrome c oxidase subunit 1 (CO1) gene (approximately 660 basepair region)
• Complies with American National Standards Institute (ANSI) guidelines for species identification of animal cell lines
• Appropriate for less commonly-used species of origin cell lines such as Drosophila, cow, pig, Syrian hamster, ferret, or other insect and mammalian species.

Interspecies Contamination

• Checks for unique markers to identify any cross-contamination between different cell lines.
• Complies with ANSI guidelines for species identification of animal cell lines.

Sample Submission Guidelines and Shipping Instructions

Our technical support team will be happy to work with you to confirm the details of your order. Please contact our specialists at quotes@abmgood.com to setup your project.

h-IMPACT Human Pathogen Testing Services – Service I

IMPACT Contamination Detection Services- Service I

Mycoplasma Detection Service