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abm Research CRO Custom Services Gene Expression Assay Service
Gene Expression Assay Service
abm’s Gene Expression Assay Service utilizes RT-PCR technology, with gene-specific primers designed to provide an expression profile for your relevant target genes. Each service includes primer & cDNA synthesis, PCR assay setup, and data analysis. Samples can be submitted as either:
- Total RNA shipped on dry ice, or
- Cell pellets shipped on dry ice (Custom RNA Isolation Service will apply per sample; Cat# C207, $450)
A certificate of analysis will be provided with each service.
“We are quite happy with the knockout cells that you made. This was a tricky knockout and hemizygous removal was much more prevalent than the double knockout, but you did it. In addition to your excellent genomic sequence characterization of both mutant LIF alleles, I have confirmed in our lab that the tumor cells have lost all mouse LIF production”
Dr. Robert Jackman, Boston University, Mouse LIF CRISPR Knock Out C26 Tumor Cell Line Generation and Screening Service
Additional Resources:
Service Details
| SERVICES | UNIT | CAT. NO. |
|---|---|---|
| Gene Expression Assay Service | 1 Marker | C138 |
If the gene of interest is a common cell type marker (listed above), abm can design the primers free of charge.
Additional Info
Common Cell Type Makers(free primer design service included)
| CELL TYPE | MARKER |
|---|---|
| Epithelial | CK18, CK19, Ep-CAM |
| Endothelial | vWF, VE-Cadherin |
| Smooth Muscle | SMα-actin |
| Fibroblast | Vimentin |
Sample Submission Guideline
| Total RNA | Minimum 2 ug / marker |
| Cell Pellets | Minimum 1 million cells / marker |
| CELL TYPE | MARKER |
|---|---|
| Epithelial | CK18, CK19, Ep-CAM |
| Endothelial | vWF, VE-Cadherin |
| Smooth Muscle | SMα-actin |
| Fibroblast | Vimentin |
| Total RNA | Minimum 2 ug / marker |
| Cell Pellets | Minimum 1 million cells / marker |
Documents
FAQs
Do I have to design or send the primers to detect my marker of interest?
If the gene of interest is a common cell type marker (listed above), then we can design the primers free of charge. If there is a specific gene of interest to be detected, we would prefer that the customer sends us the sequences of validated primers. Customers do not need to send in the physical primers, but simply provide their sequence(s). If we need to design specific primers as part of the service, additional charges may apply and the lead time will be extended.
If I am providing primer designs, how many primer designs can I provide?
Customers can provide a maximum of 2 primer designs per marker. If you would like us to test additional primers, additional charges will apply.
Citations
