You will receive two vials that are Mycoplasma-absent, as tested by our standard PCR-based method for Mycoplasma detection. Additional vials will cost extra. Note: Our Mycoplasma detection method is qualitative and thus, detection limit refers to presence or absence of PCR product as seen on an agarose gel. If Mycoplasma is absent, no amplified PCR product should be seen.

We use a PCR method to test for the presence of Mycoplasma (Cat. No. G238). This kit detects over 200 species of Mycoplasma and we do consecutive testings to make sure that the Mycoplasma does not return after treatment stops. We can also have a third party certify that the cells are free of Mycoplasma, at an additional charge.

Certainly. The link to the detection kit is http://www.abmgood.com/PCR-Mycoplasma-Detection-Kit-G238.html. This kit can detect over 200 different types of Mycoplasma species.

abm has several methods of treating Mycoplasma-contaminated samples (e.g. potent antibiotics cocktail or non-antibiotics-based treatment). Our methods show no toxicity to most cells, as determined by gene array assay.

We monitor the cells for 2 weeks after we stop treatment to ensure that the Mycoplasma contamination does not re-occur. Even though our Mycoplasma treatment has shown a very high success rate, so far no antibiotic can effect permanent removal of all Mycoplasma, as some species may become resistant to the treatment. We guarantee the absence of Mycoplasma in treated samples within our detection limit using a PCR-based detection method. In such cases of extensive Mycoplasma contamination, we recommend to use anti-Mycoplasma reagents for prevention if you cannot replace the cells, and constant monitoring of Mycoplasma levels. There are some genetic approaches reported in literature that can be tried in such cases.

Yes, we can offer Additional Vials of Delivered Cells (Cat. No. C144).