HOME 
abm Cell Biology Stem Cells and iPSCs Ready to us Stem Cell LIF-Dependent Porcine iPSCs
LIF-Dependent Porcine iPSCs
| CAT.NO | UNIT |
|---|---|
| T2503 | 1×106 cells / 1.0 ml |
| Description | Leukemia inhibitory factor-dependent induced pluripotent stem cells were derived from the inner cell mass of porcine blastocysts by up-regulating expression of KLF4 and POU5F1. They are dependent on leukemia inhibitory factor signaling for maintenance of pluripotency; can be cultured over extended passage; and have the ability to form teratomas. Resulting colonies resemble mouse embryonic stem cells in terms of colony morphology; cell cycle interval; transcriptome; and the expression of pluripotent markers. This cell line was created by lentiviral transduction with hKLF4 and M2rtTA virus. |
|---|---|
| SKU | T2503 |
| Species | Pig (Porcine) |
| Tissue/Organ/Organ System | Embryo |
| Cell Morphology | polygonal |
| Applications | For Research Use Only |
| Seeding Density | 10,000 - 20,000 cells/cm2 |
| Cell Type | Ready-to-Use Stem Cells |
| Growth Properties | Adherent |
| Expression Profile | Pluripotent markers; such as POU5F1; SOX2; and surface marker SSEA1. |
| Subculture Protocol | 1:10 split; Accutase (Millipore) used |
| Unit quantity | 1x106 cells / 1.0 ml |
| QC | 1) Reverse transcriptase PCR; 2) Real time PCR; 3) Transcriptional profiling by microarray; 4) Alkaline phosphatase staining; 5) Immunofluorescence; 6) Western blot; 7) Karyotyping |
Supporting Protocol
MSDS
QC
Other
There are no FAQs for this product yet!
There are no references for this product yet!
