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abm Cell Biology Stem Cells and iPSCs Ready to us Stem Cell Integration-free wt1 iPSC
Integration-free wt1 iPSC
| CAT.NO | UNIT |
|---|---|
| T2512 | 1×106 cells / 1.0 ml |
| Description | Generated from human fibroblasts; Integration-free Wt1 iPSC is a clonal hiPSC line resulting from Sendai-virus based reprogramming. They have been validated to have a normal karyotype; lack any viral transgenes; show hESC-like expression profile; and display full differentiation competence into derivatives of the three germ layers. This cell line; originally created for use in human cardiac disease research; is suitable for disease modelling. |
|---|---|
| SKU | T2512 |
| Species | Human (H. sapiens) |
| Tissue/Organ/Organ System | Skin |
| Cell Morphology | polygonal |
| Applications | For Research Use Only |
| Seeding Density | 20,000 cells/cm2 |
| Donor Gender | Male |
| Donor Gender | Male |
| Donor Age | Newborn |
| Cell Type | Ready-to-Use Stem Cells |
| Growth Properties | Adherent |
| Expression Profile | Normal karyotype; lack any viral transgenes; hESC-like expression profile |
| Preservation Protocol | 40% hPSC culture medium; 50% Knockout Serum Replacement (Thermo); 10% DMSO; 10 uM Y-27632; 10 ng/ml FGF2 - or hPSC freezing media from commercial suppliers |
| Unit quantity | 1x106 cells / 1.0 ml |
| QC | 1) Karyotyping; 2) RT-PCR; 3) Illumina bead arrays for whole transcriptome analysis |
Supporting Protocol
MSDS
QC
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