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abm Cell Biology Stem Cells and iPSCs Ready to us Stem Cell Chicken iPSC
Chicken iPSC
| CAT.NO | UNIT |
|---|---|
| T2502 | 1×106 cells / 1.0 ml |
| Description | Derived from chicken embryonic fibroblasts after lentiviral transduction with human stem cell genes; the Chicken iPSC line was developed as a serum-free; suspension cell line for use as an alternative to egg-based methods in viral vaccine research. Eliminating the need for trypsinization and anchorage support; suspension cells are preferable for vaccine production. In addition to decreasing overall production cost; serum-free cell expansion eliminates a potential source for viral or prion contamination; an important consideration for work in vaccine production. |
|---|---|
| SKU | T2502 |
| Species | Chicken (Galline) |
| Tissue/Organ/Organ System | Embryo |
| Applications | For Research Use Only |
| Seeding Density | 7 x 105 cells/ml |
| Population Doubling Time | Approximately 21 hours |
| Cell Type | Ready-to-Use Stem Cells |
| Growth Properties | Suspension |
| Expression Profile | lentiviral transduction utilizing the viPC kit (Thermo Scientific) with viruses containing the human stem cell genes POU5F1; NANOG; SOX2; LIN28; KLF4 and C-MYC under the promoter of human elongation factor-1 alpha (EF1a) |
| Subculture Protocol | Plate at 7 x 105 cells/mL and incubate on a shaker agitated at 50-100 rpm |
| Unit quantity | 1x106 cells / 1.0 ml |
| QC | 1) PCR |
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