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EOS Lentivirus (PL-SIN-EOS-S(4)-EiP)

CAT.NO UNIT
LVP028859 2 x 200 µl

Description This ready-to-use lentivirus is part of abm’s Lentiviral Expression System and can be used to overexpress your gene of interest in a wide range of host cells. Lentiviruses have the ability to integrate into the host genome and generate a stable cell line expressing your gene of interest.
SKU LVP028859
iPSCS Factor Type Lentivirus
iPSC Factor EOS
Vector Map PL-SIN-EOS-S(4)-EiP
Vector Size 8562
Titer >107 IU/ml
Storage Condition Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles.
Unit quantity 2 x 200 µl

Supporting Protocol
MSDS
QC
Other

Minicircles have lower transfection efficiency than infection with viral particles. However, minicircles are safer for the cells because they do not have additional non-coding components.

Minicircle DNA products are provided as 100ug DNA at 0.5ug/ul concentration (200 ul total volume).

LV028873 PL-SIN-EOS-C(3+)-EiP Lentivirus Includes C(3+), trimer of CR4 enhancer LV028874 PL-SIN-EOS-S(4+)-EiP Lentivirus Includes S(4+), tetramer of SRR2 enhancer

There are several papers referencing a number of methods of iPSC generation, these have been included below for more information. Further to this, we can offer no standard protocol for using these plasmids, and this will need to be optimized and determined by the end user in all cases. 1. Liao J, Wu Z, Wang Y, et al. Enhanced efficiency of generating induced pluripotent stem (iPS) cells from human somatic cells by a combination of six transcription factors. Cell Res. 2008;18:600-603. 2. Dowey SN, Huang X, Chou BK, et al. Generation of integration-free human induced pluripotent stem cells from postnatal blood mononuclear cells by plasmid vector expression. Nat Protoc. 2012;7:2013-2021. 3. Meng X, Neises A, Su R-J, et al. Efficient Reprogramming of Human Cord Blood CD34+ Cells Into Induced Pluripotent Stem Cells With OCT4 and SOX2 Alone. Mol Ther. 2012;20:408-416. 4. Su R-J, Baylink DJ, Neises A, et al. Efficient Generation of Integration-Free iPS Cells from Human Adult Peripheral Blood Using BCL-XL Together with Yamanaka Factors. PLoS One. 2013;8:e64496. 5. Okita K, Yamakawa T, Matsumura Y, et al. An efficient nonviral method to generate integration-free human-induced pluripotent stem cells from cord blood and peripheral blood cells. Stem Cells. 2013;31:458-466.

All commonly used bacterial strains (such as DH5alpha) can be used to propagate these plasmids, please also review the vector information/map for the corresponding resistance marker.

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