HRP conjugated affinity Purified SARS-COV-2 (Covid-19, 2019-nCov) Spike S2 Domain Antibody

HRP conjugate of SARS-CoV-2 (COVID-19, 2019-nCoV) Spike (S2) antibody. Provided at 1mg/ml (100ul)

Figure 1 ELISA test of chicken anti- COVID-19 spike S2 protein.

Antibodies: SARS-CoV-2 (COVID-19, 2019-nCoV) Spike protein was coated at dilutions ranging from 2000ng/m1 to 15ng/m1(S2) PBS. After blocking and washing, ab-131-294-HRP was incubated on the plate at 1ug/ml for 1 hour at room temperature. After a final wash, TMB was added for 10 minutes and stopped by the addition of 0.2M sulphuric acid. OD450 was measured

SPECIFICATIONS

• HOST SPECIES: Chicken
• SPECIES REACTIVITY: Virus
• HOMOLOGY: Predicted reactivity based on immunogen sequence: SARS-CoV Spike protein (S2): (100%)
• IMMUNOGEN: Anti-SARS-CoV-2 (COVID-19, 2019-nCoV) Spike antibody was raised against recombinant SARS-CoV-2 (2019-nCoV) spike protein (S2) domain. The immunogen is the spike protein S2 domain produced using HEK293 cells.

Immunogen has been tested for activity and can bind with human ACE2 in functional ELISA assay.

• TESTED APPLICATIONS: ELISA
• APPLICATIONS:  SARS-CoV-2 (COVID-19, 2019-nCoV) Spike antibody can be used for detection of full length SARS-CoV-2 (COVID-19, 2019-nCoV) Spike protein (S1+S2) or truncated SARS-CoV-2 (COVID-19, 2019-nCoV) Spike protein S2 domain by ELISA.

PROPERTIES

• CLONALITY: Polyclonal
• ISOTYPE: IgY (18G)
• CONJUGATES: HRP
• PHYSICAL STATE: Liquid
• CONCENTRATION: IgY derived from eggs at a concentration of 1mg/m1 in PBS with no preservative
• VOLUME: 100µl
• STORAGE CONDITIONS: SARS-CoV-2 (COVID-19, 2019-nCoV) Spike antibody can be stored at 4°C for one month and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ADDITIONAL INFORMATION

• ALTERNATE NAMES: SARS-CoV-2 (COVID-19, 2019-nCoV) Spike Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein
• ACCESSION NO.: QHD43416
• PROTEIN GI NO.: 1791269090

Product Specifications