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5 Kits – COVID-19 Spike Capture and Detection (HRP conjugate) ELISA pair with calibrator

CATALOG NUMBER :          ab-131-295-PAIR

This product is a SARS-CoV-2 (COVID-19, 2019-nCOV) Spike S2 domain Antibody.

 

host species – chicken, raised against recombinant SARS-CoV-2 (COVID-19).

Antibody pair for SARS-CoV-2 Spike protein (COVID-19, 2019-nCoV). Capture is rabbit anti-S1 spike protein domain. Detection is HRP-conjugate of chicken anti-S2 spike protein domain.

 

Sufficient material for: 5 X 96 well plates.

 

 

Catalog ab-131-295-PAIR

 

 

Figure 1: ELISA test COVID-19 spike protein using antibody pair ab-131-295-PAIR. Antibodies: Rabbit anti-spike protein S1 was coated at a dilution of 1/1000 in PBS for 1 hour at room temperature. After blocking, calibrator was diluted from 2000ng/ml to 15ng/m1 in blocking buffer and incubated for 1 hour on coated plate. After washing detection antibody (Chicken anti-spike protein S2 – HRP) was diluted 1/1000 in blocking buffer and incubated on the calibrator wells for 1 hour. After a final wash and tamping, the ELISA was developed by the addition of 100u1TMB to each well and stopped after 15 minutes by the addition of 0.2M sulfuric acid.

 

SPECIFICATIONS
  • HOST SPECIES: Capture: Rabbit – Detection: Chicken
  • SPECIES REACTIVITY: SARS-CoV-2 spike protein.
  • HOMOLOGY: Predicted reactivity based on immunogen sequence: SARS-CoV Spike protein (52): (100%)
  • IMMUNOGEN: Anti-SARS-CoV-2 (COVID-19, 2019-nCoV) Capture antibody was raised against recombinant SARS-CoV-2 (2019- nCoV) spike protein (S1) domain. Detection antibody was raised against recombinant SARS-CoV-2 (2019- nCoV) spike protein (S2) domain.
 
TESTED APPLICATIONS: ELISA
Calibrator supplied: SARS-CoV-2 (COVID-19, 2019-nCoV) Spike protein (17uL©250ug/m1). Full length extracellular domain with C terminal HIS affinity tag. Produced by over expression in insect cell line and binding the ACE2 confirmed by ligand binding assay.
 
 
HANDLING INFORMATION
  • Anti-S1 (Capture) formulation:  Sterile filtered in PBS. No preservatives
  • Anti-S2-HRP (Detection) formulation: Sterile filtered in PBS. No preservatives
  • Calibrator (Spike protein) formulation: 20mM Tris, 300mM NaCI, 10% Glycerol, 5% Trehalose, 5% Mannitol, 0.01% Tween-80. pH 8.0

 

Product Specifications

 

  • TARGET PROTEIN : COVID

 

  • ALTERNATIVE NAME OF TARGET : SARS-CoV-2

 

  • SIZE : 96 well

 

  • CLONALITY : polyclonal

 

  • ISOTYPE : IgY

 

  • HOST SPECIE(S) : capture – rabbit, detection – chicken

 

  • STORAGE CONDITIONS : 3 months and -20°C for one year

 

  • SAFETY WARNINGS AND PRECAUTIONS : Research Use Only. Follow instructions.