Custom Adeno-Associated Virus (AAV) Service
Recombinant Adeno-Associated Virus (AAV) is the most promising candidate for gene therapy among all viral vectors owing to its non-integrating nature and lack of pathogenicity in animal models. It is also a powerful tool in regenerative medicine due to its broad tropism and ability to transduce both proliferating and quiescent cells.
Tissue specificity of AAV can be achieved through the use of different AAV serotype capsids. abm offers packaged AAV virus as purified stock, ideal for in vivo experiments, or a crude virus preparation for in vitro cell culture experiments. Whether you are looking for over-expression or knockdown, we have it all!
Advantages of AAV:
- Does not elicit significant immune responses in vivo
- Broad tropism – tissue specificity with different AAV serotypes
- No integration into the host genome
- Ability to transduce both proliferating and quiescent cells
- Long-term expression in non-dividing cells
Testimonial:
“If I have any special needs or concerns, they are always dealt with by the Technical Support Team. I have always been pleased with the product and will continue to use this service for my research.”
John Teem, Florida Department of Agriculture & Consumer Services
Our AAV infectivity Data Speaks for Itself
Service Details
Custom AAV Plasmid Cloning
| SERVICE NAME | SIZE | CAT. NO. |
| Custom AAV plasmid cloning with compatible insert | 1 Service | C234 |
| Custom AAV plasmid cloning without compatible insert | 1 Service | C235 |
*For these services, the customer must provide a gene insert under 3kb. Please contact info@itsbio.co.kr for more information on available cloning vectors and restriction enzyme sites to qualify for the compatible insert service.
Custom AAV Production without DNA Amplification
| SERVICE NAME | SIZE | CAT. NO. |
| Custom AAV without DNA amplification (10^9 GC/ml) (AAV1) | 5 x 200 μl | C239 |
| Custom AAV without DNA amplification (10^9 GC/ml) (AAV2) | 5 x 200 μl | C240 |
| Custom AAV without DNA amplification (10^9 GC/ml) (AAV3) | 5 x 200 μl | C290 |
| Custom AAV without DNA amplification (10^9 GC/ml) (AAV4) | 5 x 200 μl | C291 |
| Custom AAV without DNA amplification (10^9 GC/ml) (AAV5) | 5 x 200 μl | C241 |
| Custom AAV without DNA amplification (10^9 GC/ml) (AAV6) | 5 x 200 μl | C242 |
| Custom AAV without DNA amplification (10^9 GC/ml) (AAV7) | 5 x 200 μl | C243 |
| Custom AAV without DNA amplification (10^9 GC/ml) (AAV8) | 5 x 200 μl | C244 |
| Custom AAV without DNA amplification (10^9 GC/ml) (AAV9) | 5 x 200 μl | C245 |
| Custom AAV without DNA amplification (10^9 GC/ml) (AAV10) | 5 x 200 μl | C246 |
| Custom AAV without DNA amplification (10^9 GC/ml) (AAV11) | 5 x 200 μl | C344 |
| Custom AAV without DNA amplification (10^9 GC/ml) (AAVDJ) | 5 x 200 μl | C345 |
| Custom AAV without DNA amplification (10^9 GC/ml) (AAVDJ-8) | 5 x 200 μl | C346 |
| Custom AAV without DNA amplification (10^12 GC/ml) (AAV1) | 2 x 100 μl | C247 |
| Custom AAV without DNA amplification (10^12 GC/ml) (AAV2) | 2 x 100 μl | C248 |
| Custom AAV without DNA amplification (10^12 GC/ml) (AAV3) | 2 x 100 μl | C292 |
| Custom AAV without DNA amplification (10^12 GC/ml) (AAV4) | 2 x 100 μl | C293 |
| Custom AAV without DNA amplification (10^12 GC/ml) (AAV5) | 2 x 100 μl | C249 |
| Custom AAV without DNA amplification (10^12 GC/ml) (AAV6) | 2 x 100 μl | C250 |
| Custom AAV without DNA amplification (10^12 GC/ml) (AAV7) | 2 x 100 μl | C251 |
| Custom AAV without DNA amplification (10^12 GC/ml) (AAV8) | 2 x 100 μl | C252 |
| Custom AAV without DNA amplification (10^12 GC/ml) (AAV9) | 2 x 100 μl | C253 |
| Custom AAV without DNA amplification (10^12 GC/ml) (AAV10) | 2 x 100 μl | C254 |
| Custom AAV without DNA amplification (10^12 GC/ml) (AAV11) | 2 x 100 μl | C488 |
| Custom AAV without DNA amplification (10^12 GC/ml) (AAVDJ) | 2 x 100 μl | C489 |
| Custom AAV without DNA amplification (10^12 GC/ml) (AAVDJ-8) | 2 x 100 μl | C490 |
| Custom AAV without DNA amplification (10^13 GC/ml) (AAV1) | 2 x 100 μl | C255 |
| Custom AAV without DNA amplification (10^13 GC/ml) (AAV2) | 2 x 100 μl | C256 |
| Custom AAV without DNA amplification (10^13 GC/ml) (AAV3) | 2 x 100 μl | C294 |
| Custom AAV without DNA amplification (10^13 GC/ml) (AAV4) | 2 x 100 μl | C295 |
| Custom AAV without DNA amplification (10^13 GC/ml) (AAV5) | 2 x 100 μl | C257 |
| Custom AAV without DNA amplification (10^13 GC/ml) (AAV6) | 2 x 100 μl | C258 |
| Custom AAV without DNA amplification (10^13 GC/ml) (AAV7) | 2 x 100 μl | C259 |
| Custom AAV without DNA amplification (10^13 GC/ml) (AAV8) | 2 x 100 μl | C260 |
| Custom AAV without DNA amplification (10^13 GC/ml) (AAV9) | 2 x 100 μl | C261 |
| Custom AAV without DNA amplification (10^13 GC/ml) (AAV10) | 2 x 100 μl | C262 |
| Custom AAV without DNA amplification (10^13 GC/ml) (AAV11) | 2 x 100 μl | C491 |
| Custom AAV without DNA amplification (10^13 GC/ml) (AAVDJ) | 2 x 100 μl | C492 |
| Custom AAV without DNA amplification (10^13 GC/ml) (AAVDJ-8) | 2 x 100 μl | C493 |
| Custom AAV Crude Preparation without DNA amplification (10^12 GC/ml) (AAV1) | 2 x 100 μl | C453 |
| Custom AAV Crude Preparation without DNA amplification (10^12 GC/ml) (AAV2) | 2 x 100 μl | C454 |
| Custom AAV Crude Preparation without DNA amplification (10^12 GC/ml) (AAV3) | 2 x 100 μl | C455 |
| Custom AAV Crude Preparation without DNA amplification (10^12 GC/ml) (AAV4) | 2 x 100 μl | C456 |
| Custom AAV Crude Preparation without DNA amplification (10^12 GC/ml) (AAV5) | 2 x 100 μl | C457 |
| Custom AAV Crude Preparation without DNA amplification (10^12 GC/ml) (AAV6) | 2 x 100 μl | C458 |
| Custom AAV Crude Preparation without DNA amplification (10^12 GC/ml) (AAV7) | 2 x 100 μl | C459 |
| Custom AAV Crude Preparation without DNA amplification (10^12 GC/ml) (AAV8) | 2 x 100 μl | C460 |
| Custom AAV Crude Preparation without DNA amplification (10^12 GC/ml) (AAV9) | 2 x 100 μl | C461 |
| Custom AAV Crude Preparation without DNA amplification (10^12 GC/ml) (AAV10) | 2 x 100 μl | C462 |
| Custom AAV Crude Preparation without DNA amplification (10^12 GC/ml) (AAV11) | 2 x 100 μl | C463 |
| Custom AAV Crude Preparation without DNA amplification (10^12 GC/ml) (AAVDJ) | 2 x 100 μl | C464 |
| Custom AAV Crude Preparation without DNA amplification (10^12 GC/ml) (AAVDJ-8) | 2 x 100 μl | C465 |
To qualify for Custom AAV Production without DNA amplification, the following amounts of the AAV vector containing the gene of interest must be supplied at >0.5μg/μl:
- 10^9 GC/ml: 75 μg
- 10^12 GC/ml: 250 μg
- 10^13 GC/ml: 750 μg
For customer-supplied vectors, the customer must indicate prior to the service if any reporter expression will be expected or not. The expected reporter expression, e.g. GFP, should not require induction of expression.
Custom AAV Production with DNA Amplification
| SERVICE NAME | SIZE | CAT. NO. |
| Custom AAV with DNA amplification (10^9 GC/ml) (AAV1) | 5 x 200 μl | C263 |
| Custom AAV with DNA amplification (10^9 GC/ml) (AAV2) | 5 x 200 μl | C264 |
| Custom AAV with DNA amplification (10^9 GC/ml) (AAV3) | 5 x 200 μl | C296 |
| Custom AAV with DNA amplification (10^9 GC/ml) (AAV4) | 5 x 200 μl | C297 |
| Custom AAV with DNA amplification (10^9 GC/ml) (AAV5) | 5 x 200 μl | C265 |
| Custom AAV with DNA amplification (10^9 GC/ml) (AAV6) | 5 x 200 μl | C266 |
| Custom AAV with DNA amplification (10^9 GC/ml) (AAV7) | 5 x 200 μl | C267 |
| Custom AAV with DNA amplification (10^9 GC/ml) (AAV8) | 5 x 200 μl | C268 |
| Custom AAV with DNA amplification (10^9 GC/ml) (AAV9) | 5 x 200 μl | C269 |
| Custom AAV with DNA amplification (10^9 GC/ml) (AAV10) | 5 x 200 μl | C270 |
| Custom AAV with DNA amplification (10^9 GC/ml) (AAV11) | 5 x 200 μl | C347 |
| Custom AAV with DNA amplification (10^9 GC/ml) (AAVDJ) | 5 x 200 μl | C348 |
| Custom AAV with DNA amplification (10^9 GC/ml) (AAVDJ-8) | 5 x 200 μl | C349 |
| Custom AAV with DNA amplification (10^12 GC/ml) (AAV1) | 2 x 100 μl | C271 |
| Custom AAV with DNA amplification (10^12 GC/ml) (AAV2) | 2 x 100 μl | C272 |
| Custom AAV with DNA amplification (10^12 GC/ml) (AAV3) | 2 x 100 μl | C298 |
| Custom AAV with DNA amplification (10^12 GC/ml) (AAV4) | 2 x 100 μl | C299 |
| Custom AAV with DNA amplification (10^12 GC/ml) (AAV5) | 2 x 100 μl | C273 |
| Custom AAV with DNA amplification (10^12 GC/ml) (AAV6) | 2 x 100 μl | C274 |
| Custom AAV with DNA amplification (10^12 GC/ml) (AAV7) | 2 x 100 μl | C275 |
| Custom AAV with DNA amplification (10^12 GC/ml) (AAV8) | 2 x 100 μl | C276 |
| Custom AAV with DNA amplification (10^12 GC/ml) (AAV9) | 2 x 100 μl | C277 |
| Custom AAV with DNA amplification (10^12 GC/ml) (AAV10) | 2 x 100 μl | C278 |
| Custom AAV with DNA amplification (10^12 GC/ml) (AAV11) | 2 x 100 μl | C494 |
| Custom AAV with DNA amplification (10^12 GC/ml) (AAVDJ) | 2 x 100 μl | C495 |
| Custom AAV with DNA amplification (10^12 GC/ml) (AAVDJ-8) | 2 x 100 μl | C496 |
| Custom AAV with DNA amplification (10^13 GC/ml) (AAV1) | 2 x 100 μl | C279 |
| Custom AAV with DNA amplification (10^13 GC/ml) (AAV2) | 2 x 100 μl | C280 |
| Custom AAV with DNA amplification (10^13 GC/ml) (AAV3) | 2 x 100 μl | C300 |
| Custom AAV with DNA amplification (10^13 GC/ml) (AAV4) | 2 x 100 μl | C301 |
| Custom AAV with DNA amplification (10^13 GC/ml) (AAV5) | 2 x 100 μl | C281 |
| Custom AAV with DNA amplification (10^13 GC/ml) (AAV6) | 2 x 100 μl | C282 |
| Custom AAV with DNA amplification (10^13 GC/ml) (AAV7) | 2 x 100 μl | C283 |
| Custom AAV with DNA amplification (10^13 GC/ml) (AAV8) | 2 x 100 μl | C284 |
| Custom AAV with DNA amplification (10^13 GC/ml) (AAV9) | 2 x 100 μl | C285 |
| Custom AAV with DNA amplification (10^13 GC/ml) (AAV10) | 2 x 100 μl | C286 |
| Custom AAV with DNA amplification (10^13 GC/ml) (AAV11) | 2 x 100 μl | C497 |
| Custom AAV with DNA amplification (10^13 GC/ml) (AAVDJ) | 2 x 100 μl | C498 |
| Custom AAV with DNA amplification (10^13 GC/ml) (AAVDJ-8) | 2 x 100 μl | C499 |
| Custom AAV Crude Preparation with DNA amplification (10^12 GC/ml) (AAV1) | 2 x 100 μl | C466 |
| Custom AAV Crude Preparation with DNA amplification (10^12 GC/ml) (AAV2) | 2 x 100 μl | C467 |
| Custom AAV Crude Preparation with DNA amplification (10^12 GC/ml) (AAV3) | 2 x 100 μl | C468 |
| Custom AAV Crude Preparation with DNA amplification (10^12 GC/ml) (AAV4) | 2 x 100 μl | C469 |
| Custom AAV Crude Preparation with DNA amplification (10^12 GC/ml) (AAV5) | 2 x 100 μl | C470 |
| Custom AAV Crude Preparation with DNA amplification (10^12 GC/ml) (AAV6) | 2 x 100 μl | C471 |
| Custom AAV Crude Preparation with DNA amplification (10^12 GC/ml) (AAV7) | 2 x 100 μl | C472 |
| Custom AAV Crude Preparation with DNA amplification (10^12 GC/ml) (AAV8) | 2 x 100 μl | C473 |
| Custom AAV Crude Preparation with DNA amplification (10^12 GC/ml) (AAV9) | 2 x 100 μl | C474 |
| Custom AAV Crude Preparation with DNA amplification (10^12 GC/ml) (AAV10) | 2 x 100 μl | C475 |
| Custom AAV Crude Preparation with DNA amplification (10^12 GC/ml) (AAV11) | 2 x 100 μl | C476 |
| Custom AAV Crude Preparation with DNA amplification (10^12 GC/ml) (AAVDJ) | 2 x 100 μl | C477 |
| Custom AAV Crude Preparation with DNA amplification (10^12 GC/ml) (AAVDJ-8) | 2 x 100 μl | C478 |
*To qualify for Custom AAV Production with DNA amplification, 10μg of the AAV vector containing the gene of interest must be supplied, along with a plasmid map and full sequence for plasmid amplification. For customer supplied vectors, the customer must indicate prior to the service if any reporter expression will be expected or not. The expected reporter expression, e.g. GFP, should not require induction of expression.
Custom siRNA AAV Production
| SERVICE NAME | SIZE | CAT. NO. |
| AAV siRNA Pooled Virus (AAV1)(10^9 GC/ml) | 5 x 200 μl | iAAV04401 |
| AAV siRNA Pooled Virus (AAV2)(10^9 GC/ml) | 5 x 200 μl | iAAV04402 |
| AAV siRNA Pooled Virus (AAV3)(10^9 GC/ml) | 5 x 200 μl | iAAV04403 |
| AAV siRNA Pooled Virus (AAV4)(10^9 GC/ml) | 5 x 200 μl | iAAV04404 |
| AAV siRNA Pooled Virus (AAV5)(10^9 GC/ml) | 5 x 200 μl | iAAV04405 |
| AAV siRNA Pooled Virus (AAV6)(10^9 GC/ml) | 5 x 200 μl | iAAV04406 |
| AAV siRNA Pooled Virus (AAV7)(10^9 GC/ml) | 5 x 200 μl | iAAV04407 |
| AAV siRNA Pooled Virus (AAV8)(10^9 GC/ml) | 5 x 200 μl | iAAV04408 |
| AAV siRNA Pooled Virus (AAV9)(10^9 GC/ml) | 5 x 200 μl | iAAV04409 |
| AAV siRNA Pooled Virus (AAV10)(10^9 GC/ml) | 5 x 200 μl | iAAV04410 |
| AAV siRNA Pooled Virus (AAV11)(10^9 GC/ml) | 5 x 200 μl | C500 |
| AAV siRNA Pooled Virus (AAVDJ)(10^9 GC/ml) | 5 x 200 μl | C501 |
| AAV siRNA Pooled Virus (AAVDJ-8)(10^9 GC/ml) | 5 x 200 μl | C502 |
| Custom AAV siRNA Pooled Vector | 1.0 μg | iAAV04400 |
Titer Upgrades for Existing AAV Viruses
| SERVICE NAME | SIZE | CAT. NO. |
| Upgrade from 10^9 AAV Virus to High Titer AAV Virus (10^12 GC/ml) | 2 x 100 μl | C288 |
| Upgrade from 10^9 AAV Virus to High Titer AAV Virus (10^13 GC/ml) | 2 x 100 μl | C289 |
| Upgrade from 10^9 AAV Virus to High Titer Crude Preparation AAV Virus (10^12 GC/ml) | 2 x 100 μl | C479 |
AAV Cell Line
| SERVICE NAME | SIZE | CAT. NO. |
| AAV Packaging Cell Line for High Titer Virus Production | 10^6 Cells | C236 |
Custom AAV Titration
| SERVICE NAME | SIZE | CAT. NO. |
| Custom AAV Titration using qPCR Assay | 1 Service | C238 |
AAV Virus Custom Aliquoting
| SERVICE NAME | SIZE | CAT. NO. |
| AAV Virus Custom Aliquoting | Up to 10 Vials | C506 |
*Minimum volume per vial = 20 μl
Additional Info
Don’t Know Which Serotype To Use?
Related Products
FAQs
- Is your recombinant AAV replication-deficient?
Yes, the replication and capsid genes are provided in trans when the AAV is produced, therefore the packaged virion only has the ITR sequences and the gene of interest. Furthermore, the cis plasmid and rep/cap plasmid do not share any regions of homology, preventing the production of wild-type AAV through recombination system.
- What is the packaging capacity for AAV?
The maximum insert size is < 4.4 kb.
- Is AAV stable? What is the recommended storage temperature?
It is recommended to store AAV at -80˚C for long-term storage. For short-term, AAV is stable at 4˚C for up to three weeks without significant loss of activity.
- How much AAV virus should be submitted for the Custom AAV Titration service, Cat# C238?
Please send at least 50μl of virus if it is low titer. If it is expected to be high titer AAV virus, please send at least 20μl of sample.
Citations
| 01 |
Zhang, Y et al. “Hypothalamic stem cells control ageing speed partly through exosomal miRNAs..” Nature 548(7665):52-57 (2017). DOI: 10.1038/nature23282 PubMed: 28746310 Application: In vivo infection.
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