Porducts
Can be used to synthesize stable isotope labeled proteins
His-tagged Protein Recommendation
Protein Expression Kit
WEPRO8240H Core Kit
WEPRO8240H Core Kit
Comes as a set with reagents necessary for stable isotope labeled proteins synthesis.
Synthesises by adjusting stable isotope labeled proteins, according to the customer’s goals.
Improved removal performance against His-like proteins composed of wheat germ.
Exhibits a higher level of His-tagged fusion protein purity compared to WEPRO8240 Core Kit.
* This kit does not come with isotope labeled amino acids included.
If the customer wants to prepare and adjust amino acids themselves, our order-made translation buffer can be used, requiring no separate amino acid adjustment.
Features:
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Can be used for 4 reactions on 6ml reaction scale, 20 reactions on 1.2 ml reaction scale and 96 reactions on 226μl reaction scale.
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Target proteins can be confirmed through SDS-PAGE/CBB staining.
Recommended for:
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Users who require yields of soluble protein ranging from a few hundred micrograms to several miligrams.
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Users who want to progress with the synthesis of dozens of samples as soluble protein simultaneously.
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Users who want to perform screening.
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Users who want to perform structure analysis.
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Users who want to perform interaction analysis.
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Users who want to perform function analysis.
Flow of operations
Performs purification and determines expression, solubility, activity, etc. of the target protein according to the customer’s needs.
Specification
| Product Number | Product Name | Size | Instruction manual | Safety Data Sheet | |
|---|---|---|---|---|---|
| CFS-C8H | WEPRO8240H Core Kit | 1 set |  |
Product Composition
|
Reagent Name |
Concentration |
Volume |
Quantity |
|---|---|---|---|
|
WEPRO8240H |
240 OD |
1 mL |
1 |
| SUB-AMIX SGC NA S1* | 40x |
600 µL |
1 |
|
SUB-AMIX SGC NA S2* |
40x |
600 µL |
1 |
|
SUB-AMIX SGC NA S3* |
40x |
600 µL |
1 |
| SUB-AMIX SGC NA S4* | 40x |
600 µL |
1 |
|
Creatine Kinase |
20 mg/mL |
20 µL |
1 |
|
NTPmix |
25 mM |
120 µL |
1 |
|
5×Transcription Buffer LM |
5x |
240 µL |
1 |
|
RNase Inhibitor |
80K unit/mL |
15 µL |
1 |
|
SP6 RNA Polymerase |
80K unit/mL |
15 µL |
1 |
Storage Condition : -80 ℃
*SUB-AMIX SGC NA S1-S4 each vary in their composition.
When producing a 1x concentration, adjust by adding the same quantity of each.
