WEPRO7240H Core Kit

WEPRO7240H Core Kit

Porducts

Can be used to synthesize all types of proteins

His-tagged Protein Recommendation

Protein Expression Kit

Users can freely alter the reaction scale according to their goals and the quantity of protein required.

Perfectly suited for both small-scale synthesis of multiple samples, or large scale production of a specific protein.

Improved removal performance against His-like proteins composed of wheat germ.

Exhibits a higher level of His-tagged fusion protein purity compared to WEPRO7240 Core Kit.

Can be used for 4 reactions on 6ml reaction scale, 20 reactions on 1.2 ml reaction scale and 96 reactions on 226μl reaction scale.
Target proteins can be confirmed through SDS-PAGE/CBB staining

Users who require yields of soluble protein ranging from a few hundred micrograms to several miligrams.
Users who want to progress with the synthesis of dozens of samples as soluble protein simultaneously.
Users who want to perform screening.
Users who want to perform crystal structure analysis.
Users who want to perform interaction analysis.
Users who want to perform function analysis.
Users who want to perform complex formation (co-expression).

WEPRO7240 Expression Kit img en

Performs purification and determines expression, solubility, activity, etc. of the target protein according to the customer’s needs.

Specification

Product Number	Product Name	Size		Instruction manual	Safety Data Sheet
CFS-C7H	WEPRO7240H Core Kit	1 set

Reagent Name	Concentration	Volume	Quantity
WEPRO7240H	240 OD	1 mL	1
SUB-AMIX SGC S1*	40x	600 µL	1
SUB-AMIX SGC S2*	40x	600 µL	1
SUB-AMIX SGC S3*	40x	600 µL	1
SUB-AMIX SGC S4*	40x	600 µL	1
Creatine Kinase	20 mg/mL	20 µL	1
NTPmix	25 mM	120 µL	1
5×Transcription Buffer LM	5x	240 µL	1
RNase Inhibitor	80K unit/mL	15 µL	1
SP6 RNA Polymerase	80K unit/mL	15 µL	1

*SUB-AMIX SGC S1-S4 each vary in their composition.

When producing a 1x concentration, adjust by adding the same quantity of each.