Cell Immortalization Kits
Primary cells have a finite life span of less than 10-50 passages in vitro depending on cell type. Immortalizing your cell line will eliminate the frequent need to re-establish fresh cultures from specimens. Immortalized cell lines are capable of extended proliferation and should possess similar or identical genotype and phenotype to the parental tissue.
Immortalization Strategies for Mammalian Cells
Several methods exist for immortalizing mammalian cells in culture. With years of experience,abmhas developed the most comprehensive cell immortalization products comprising of ready-to-use retroviral, lentiviral and adenoviral vectors.
| GENE OVEREXPRESSED | VIRUSES OFFERED | APPLICATION |
|---|---|---|
| hTERT | Adenovirus, Lentivirus, Retrovirus |
Reliable and commonly used. |
| SV40 T Antigen | Adenovirus, Lentivirus, Retrovirus |
Reliable and commonly used. Often used to immortalize epithelial cells. |
| EBV genes | Epstein-Barr Virus | Immortalize B lymphocytes, and sometimes T lymphocytes. |
| HPV E6-E7 | Lentivirus | Immortalize keratinocytes. |
| HOXB8, HOXA9, HOXA10 | Lentivirus | Immortalize hematopoietic cells, including macrophages, hematopoetic progenitor cells, and myeloid progenitor cells. |
| Adenoviral E1A-E1B | Lentivirus | Immortalize a wide range of primary rodent cells, including amniotic fluid cells, primary epithelial cells, fibroblasts, and primary kidney cells. |
| p53 siRNA, Rb siRNA | Lentivirus | Co-express with hTERT for better immortalization of epithelial cells. |
| Bmi1 | Lentivirus | Immortalize epithelial cells. |
| CDK4 | Lentivirus | Co-express with hTERT for a more authentic cell model. |
| Myc T58A (c-Myc), Ras V12 (H-Ras) | Lentivirus | Immortalizes a wide variety of cells, including neural stem cells, primary kidney cells, erythroblasts, and a variety of epithelial cells (primary prostate, mammary, airway, etc.). |
Popular Products
- ViralPlus Transduction Enhancer
- GFP Control Lentivirus (Neo)
- PriCoat Flasks
- Lenti-hTERT (No Selection Marker) virus
- Immortalized Cells
Additional Resources on Cell Culture Methods
Tools & Resources
This Handbook is designed to give an overview of Cell Immortalization, Including methods, protocols, and reagents.
Overview of abm‘s Immortalization service workflow. For more information, visit our Cell Immortalization Knowledge base.
Can’t find what you are looking for? We can help!
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| Expression System Type | Adenovirus |
| Lentivirus | |
| Retrovirus | |
| Overexpressed Gene | Myc T58A (c-Myc), Ras V12 (H-Ras) |
| p53 siRNA, Rb siRNA | |
| SV40 T Antigen | |
| HPV E6-E7 | |
| hTERT | |
| Bmi1 | |
| CDK4 | |
| Adenoviral E1A-E1B | |
| HOXB8, HOXA9, HOXA10 |
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Cell Immortalization reagent Search
We offer a wide variety of cell immortalization reagents.
Can’t find the cell line you’re looking for? Contact info@itsbio.co.kr.
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