Primary antibodies are configured to recognize and bind unique regions (epitopes) that can in essence, be presented in the context of a broad range of bio-molecules. The specific nature of the interaction between a primary antibody and its associated epitope has led to the routine application of primary antibodies in the detection/quantification of target molecules in applications such as Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (Flow Cyt), Immunofluorescence (IF), and Immunoprecipitation (IP). As one of the leading suppliers of antibodies worldwide, abm provides >30,000 gene-specific antibodies in addition to the availability of both the monoclonal and polyclonal antibodies from a wide range of host sources (i.e. rabbit, mouse, donkey, goat).

Secondary antibodies are designed to specifically detect the primary antibody, and functions to assist in either the detection, sorting and purification of target antigens. A secondary antibody is specific, both at the species and isotype level, to the relevant primary antibody and usually bears a fluorescent/radioactive/ chemiluminescent or a chromogenic – tag for signal detection and quantification. abm offers a comprehensive selection in regards to species of origin (human, mice, rats, rabbits, sheep, chickens, horses, guinea pigs and more), and a wide array of labelled/conjugated (i.e. Horseradish Peroxidase, biotin, Alkaline Phosphatase and Fluorescein) secondary antibodies with high signal sensitivity and low background.

“I have been using anti-GFP antibodies from your company throughout my entire grad school at the University of Chicago so when I started at Yale I knew from my prior experience that the anti-GFP antibodies from your company are the best from all that I tested and work for various assays with high efficiency and specificity.”

Anna Luchniak, Yale University, Tag Antibody