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Nt.BstNBI (Bst Nickase)
Nt.BstNBI (Bst Nickase)
| CAT.NO | UNIT |
|---|---|
| E042 | 1000 U (100 μl) |
| Description | Nt.BstNBI (Bst Nickase) is a nicking endonuclease from Bacillus stearothermophilus that cleaves one DNA strand of a double-stranded DNA substrate. This site-specific endonuclease cleaves a single strand of DNA four base pairs away and 3’ of its recognition sequence. |
|---|---|
| SKU | E042 |
| Unit quantity | 1000 U (100 μl) |
| Applications | Single strand DNA nicking. |
| Concentration | 10 U/μl |
| Format | Enzyme supplied with 10X Reaction Buffer |
| Storage Condition | Store all components at -20°C. |
| Storage Buffer | 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 50 mM KCl, 200 µg/ml BSA and 50% (v/v) Glycerol. |
| Note | One unit is defined as the amount of Nt.BstNBI (Bst Nickase) that digests 1 µg of T7 DNA into 5 distinct bands (13, 9, 7, 6, and 4 kb) in 1 hour at 55°C in a reaction volume of 50 µl.
This product is distributed for laboratory research only. Caution: Not for diagnostic use. |
Supporting Protocol
