Exonuclease I, E. coli | ITSBio

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Exonuclease I, E. coli

CAT.NO	UNIT
E015	2000 U (100 μl)

Description	Exonuclease I, E.coli catalyzes the removal of nucleotides from single-stranded DNA in the 3’→5’ direction, releasing deoxyribonucleoside 5'-monophosphates and leaving 5'-terminal dinucleotides intact. Hydrolysis cannot proceed if the 3’-terminus is phosphorylated.
SKU	E015
Unit quantity	2000 U (100 μl)
Applications	Removal of single-stranded primer oligonucleotides from: o PCR mixtures for applications involving sequencing or labelling o from nucleic acid mixtures Assaying for the presence of single-stranded DNA with a 3'-hydroxyl terminus Note: This product is not suitable for NGS or second strand cDNA synthesis applications.
Concentration	20 U/μl
Format	Enzyme supplied with 10X Reaction Buffer
Storage Condition	Store all components at -20°C.
Storage Buffer	20 mM HEPES (pH 7.1), 150 mM NaCl, 1 mM DTT, 1 mM EDTA, and 50% (v/v) Glycerol.
Note	One unit is defined as the amount of Exonuclease I, E. coli that is required to catalyze the release of 10 nmol of acid soluble nucleotides from heat-denatured DNA in 30 minutes at 37°C in 1X Exonuclease I Reaction Buffer. This product is distributed for laboratory research only. Caution: Not for diagnostic use.

Supporting Protocol

Exonuclease I Data Sheet