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T4 DNA Polymerase

CAT.NO	UNIT
E005	500 U (100 μl)

Description	T4 DNA Polymerase catalyzes the 5’→3’ synthesis of DNA from a single stranded, primed DNA template. This high fidelity enzyme also has potent 3’→5’ exonuclease activity but lacks 5’→3’ exonuclease function. T4 DNA Polymerase is the ideal choice for creating blunt ended DNA by removing 3’-overhangs or 5’-overhang filling, and is also useful for second strand DNA synthesis in site-specific mutagenesis.
SKU	E005
Unit quantity	500 U (100 μl)
Applications	Generates blunt end DNA by filling in 5'-overhangs or/and removing 3'-overhangs High fidelity due to strong 3’→5’ exonuclease activity Synthesis of labeled DNA probes by the replacement reaction Site-specific mutagenesis via primer extension from oligonucleotides
Concentration	5 U/μl
Format	Enzyme supplied with 10X Reaction Buffer
Storage Condition	Store all components at -20°C.
Storage Buffer	100 mM KPO₄ (pH 6.5), 1 mM DTT, and 50% (v/v) Glycerol.
Note	One unit is defined as the amount of T4 DNA Polymerase that catalyzes the incorporation of 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C using poly(dA-dT):poly(dA-dT) as a template:primer. This product is distributed for laboratory research only. Caution: Not for diagnostic use.

Supporting Protocol

T4 DNA Polymerase

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