HOME 
NheI
NheI
| CAT.NO | UNIT |
|---|---|
| E064 | 5000 U (500 µl) |
| SKU | E064 |
|---|---|
| Unit quantity | 5000 U (500 µl) |
| Applications | Restriction enzyme digestion of double-stranded DNA |
| Components | NheI enzyme supplied with 10X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204) |
| Concentration | 10000 U/ml |
| Expression System | An E. coli strain that carries the cloned and modified NheI gene from Neisseria mucosa heidelbergensis (ATCC 25999). The NheI enzyme recognizes and cuts the sequence G^CTAGC. |
| Inactivation Protocol | 80°C for 20 min |
| Reaction Definition | 1X Universal Restriction Enzyme Reaction Buffer (Cat.No. E204) Incubate at 37°C |
| Reaction Buffer | Buffer 1: 100%, Buffer 2: 25%, Buffer 3: 10%, Universal Buffer: 100% Click here to view the buffer compatibility chart for restriction enzymes. |
| Storage Condition | -20°C |
| Storage Buffer | 10mM Tris pH (7.5), 250mM NaCl, 1mM DTT, 0.1mM EDTA, and 50% (v/v) Glycerol. |
| Note | Methylation Sensitivity dam methylation: Not Sensitive dcm methylation: Not Sensitive CpG Methylation: Blocked by some combinations of overlapping |
Supporting Protocol
MSDS
QC
Other
