IHC Optimization Service

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If you have a newly developed antibody that has not been tested for IHC staining yet, abm will work with you to provide the best IHC staining conditions for your antibody.


Figure 1. p53 antibody staining yielding high background and low specificity staining.	Figure 2. After IHC optimization service, p53 antibody staining yields low background and high specificity.

Before and After IHC Optimization

For any antibody used for immunohistochemistry staining, optimization of the pretreatment and working concentration is crucial to obtain the best staining results. After fixation of the tissue, proteins are cross linked to one another which prevent some epitopes from binding to the complementary antibody. To improve binding and subsequent staining of the marker of interest, different pretreatment methods are utilized to break the cross linkage of proteins. Antibody concentration will also greatly affect antibody-antigen binding and staining intensity. To obtain the best staining with minimum amount of background, the working concentration for each antibody must be determined experimentally.

“We are happy about the CD4 and CD8 IHC staining your lab did. The quality of the IHC staining is very good.”

Fugang Zhu, Idera Pharnacueticals Inc

Additional Resources:

IHC Services Flyer

Service Details

Core Services

SERVICE NAME	DESCRIPTION	CAT. NO.
IHC Optimization Service	Optimization of a specific antibody to obtain the best staining results with minimal background. To obtain the best results, 3 methods of pretreatment, and 4 different dilutions will be tested for each case. Customers to provide: (1) minimum of 30 µg of the primary antibody, and , (2) one positive control tissue block for the marker of interest Turnaround time: 2-3 weeks. Deliverables: Images of all 12 slides will be given to the customer. For delivery of actual slides, please inquire.	HC015

SERVICE NAME

DESCRIPTION

CAT. NO.

IHC Optimization Service

Optimization of a specific antibody to obtain the best staining results with minimal background. To obtain the best results, 3 methods of pretreatment, and 4 different dilutions will be tested for each case.

Customers to provide:
(1) minimum of 30 µg of the primary antibody, and , (2) one positive control tissue block for the marker of interest
Turnaround time: 2-3 weeks.

Deliverables: Images of all 12 slides will be given to the customer. For delivery of actual slides, please inquire.

HC015

Add-On Services*

Have the tissue, but can’t embed it or cut it? Don’t worry, send us the tissue sample/block, and we can do the embedding and/or cutting for you!

ADD-ON SERVICES*	CAT. NO.
Tissue Processing# Processing of Formalin-Fixed wet tissue suitable for paraffin embedding. Deliverables: Processed wet tissue suitable for paraffin embedding.	HC004
Paraffin Embedding Embedding processed tissue into FFPE block suitable for sectioning and subsequent staining. Deliverables: FFPE tissue block	HC005
Cell Pellet Processing and Embedding Processing of wet cell pellet into FFPE block suitable for sectioning and subsequent staining. Deliverables: Paraffin embedded cell pellet block	HC009
Paraffin Sectioning Sectioning of FFPE blocks to produce unstained slides. Unstained slides can be used for H&E, IHC or dye based staining. All slides are cut at 4 μM unless otherwise specified. Please inquire for Tissue Microarray sectioning. Deliverables: Unstained FFPE tissue section slides.	HC006
Serial Sectioning Multiple sections from the same tissue block cut at set distances from one another by the Microtome Deliverables: Multiple slides cut in sequence by the Microtome from the same specimen.	HC007
Step Sectioning Multiple sections from the same tissue block cut at set distances from one another by the Microtome Deliverables: Multiple slides within a sample at set distance from one another	HC008
Digital Scan Digital scans of stained slides at 10X, 20X or 40X magnification. This service is offered in conjunction with IHC, H&E or special staining services. Deliverables: Digital scan will be digitally provided to the customer.	HC016
	HC017
	HC018
Image Analysis Image analysis of stained slides using professional software. Deliverables: Analysis results will be sent digitally to the client.	HC011
Pathological Evaluation Pathological evaluation of stained slides by trained professionals. Deliverables: Evaluation report will be sent digitally to the client.	HC012

^#Minimum order of 10 tissue samples is required.

Additional Info

Sample Submission Guidelines and Shipping Instructions

Please fill out our Histology Service Request Form, and email it to quotes@abmgood.com to initiate an inquiry. Before shipping, please fill out our Histology Services Sample Submission Form with your order number and follow the shipping instructions for your samples.

SPECIMEN TYPE	SHIPPING INSTRUCTIONS
Unstained slides	Place slides in microscope slide boxes. Make sure to seal and wrap the box well.
Formalin-Fixed wet tissues	Transfer formalin-fixed sample to 70% alcohol. Seal and wrap the container well to prevent leaks.
Fixed cell pellet	Transfer the samples to 70% alcohol. Seal and wrap the container well to prevent leaks.
FFPE tissue blocks	Ship tissue blocks in a cassette case or bag. During the month of May to August, ship blocks with gel ice pack to prevent the block from melting due to higher temperatures.