Use abm’s double-floxed inverse ORF (DIO) vectors for Cre-inducible expression of your gene-of interest! The DIO system allows you to express your target gene only in cells/tissues that also express the Cre recombinase protein, allowing for tissue-specific gene expression. This system is also called Cre-ON, Cre-Switch, FLEx-Cre, Flex-rev (reverse), or Flex-ON. We offer the DIO system in AAV and Lentivector formats.

• Adeno-Associated Virus (AAV) has low immunogenicity & pathogenicity (ideal for gene therapy), can target specific tissue types by using different serotypes, and infects dividing/non-dividing cells.
• Lentiviruses can infect both dividing and non-dividing cells and will integrate stably into the host cell genome, ensuring long-term expression even in cells that are difficult to infect

We also offer high-titer packaging services as well as Cre-expression AAVs and tamoxifen-inducible Cre Lentivirus to support your DIO project from cloning to expression.

Core Services

Controls:

Interested in Lentivirus Packaging and AAV Packaging options? Please inquire with us!

Our data speaks for itself:

Lenti:

Left: 293 cells infected with Cat. No. LV661 Lenti-CMV-DIO-copGFP-SV40-Puro Vector showing copGFP expression in the presence of CRE	Right: 293 cells infected with Cat. No. LV661 Lenti-CMV-DIO-copGFP-SV40-Puro Vector only (i.e. absence of CRE)

AAV:

Left: 293 cells infected with Cat. No. AAV101 AAV-CMV-DIO-copGFP Vector showing copGFP expression in the presence of CRE	Right: 293 cells infected with Cat. No. AAV101 AAV-CMV-DIO-copGFP Vector only (i.e. absence of CRE)

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Lentivirus Packaging Service

AAV Packaging Service

Cre Lentivirus