Site Directed Mutagenesis Service

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abm has over 10 years’ experience with Site-Directed Mutagenesis Services and we have successfully completed hundreds of orders. Past projects include modifying a DNA construct, such as alteration of a restriction site in a vector, insertion or deletion of vector elements, tag addition/removal, base substitution, sequence deletion/insertion and promoter insertion/swapping. For large plasmids, large fragment insertions/deletions, difficult templates, and other mutagenesis requests, please inquire.

SERVICE
Procedure	PCR-Based Mutagenesis
Delivery Format	Plasmid DNA
Lead Time	In as little as 2-3 weeks for orders of 1.0 μg of DNA
Quality Control	Full-length sequencing to confirm presence of mutation
DNA Submission Guidelines	At least 10μg DNA per mutated clone required (200ng/μl or higher). Plasmids must be high copy plasmids. For medium or low copy plasmids, please inquire.

“Our lab has used abm‘s site-directed mutagenesis service many times, and you always produce great plasmids!”

Stuart Jarrett, University of Kentucky

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Service Details

Core Services

SERVICE NAME	UNIT	CAT. NO.
Site-Directed Mutagenesis Service	Single Clone	C047

ADD-ON-SERVICES

SERVICE NNAME	UNIT	CAT. NO.
DNA Sequencing	Single Reaction	C199
DNA Sequencing with Custom Primer Synthesis	Single Reaction	C197
Bacterial Glycerol Stock	1 clone	C139
Plasmid Amplification (10-20 μg)	10‑20 μg	C309
Plasmid Amplification (80-100 μg)	80‑100 μg	C310

Mutate up to 25bp (consecutive) within a <2kb fragment in any existing abm product or a provided vector.
*Fragments over 2kb are subject to an additional charge of $100.00/kb (C100).

Additional Info

Coming Soon

Does this also apply for insertions/deletions/mutations that span >25 consecutive base pairs?

Pricing for Cat# C047 only applies for insertions/deletions/mutations that span <25 consecutive base pairs or fewer. Additional charges may apply for any insertions/deletions/mutations above the initial 25bp.

Citations

01	Wen, Z et al. “MicroRNA-377 Regulates Mesenchymal Stem Cell-Induced Angiogenesis in Ischemic Hearts by Targeting V.” PLoS One 9(9):e104666 (2014). DOI: 10.1371/journal.pone.0104666 PubMed: 25251394 Application: Inhibitor miRNA lentivector.
02	Laryea, G et al. “Site-specific modulation of brain glucocorticoid receptor and corticotropin-releasing hormone expression using lentiviral vectors.” Mol Cell Endocrinol 371(1-2):160-5 (2013). DOI: 23261985 PubMed: 10.1016/j.mce.2012.12.005 Application: EF1a lentivector.
03	Das, A et al. “Engulfment of apoptotic cells by macrophages: a role of microRNA-21 in the resolution of wound inflammation..” J Immunol 192(3):1120-9 (2014). DOI: 10.4049/jimmunol.1300613 PubMed: 24391209 Application: Transduction.