Inorganic Pyrophosphatase, Thermostable
CAT.NO | UNIT |
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E021 | 100 μl |
Description | Inorganic pyrophosphatase, Thermostable catalyzes the hydrolysis reaction of inorganic pyrophosphate to orthophosphate. P2O7-4 H2O -> 2HPO4-2. |
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SKU | E021 |
Unit quantity | 100 μl |
Concentration | 2U/ul |
Expression System | The source of this protein is a recombinant E. coli strain encoding pyrophosphatase from Thermococcus litoralis. |
Unit Definition | One unit is defined as the amount of enzyme needed to generate 1 μmol of phosphate in 1 minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 75°C in 50 mM Tricine [pH 8.5] , 1 mM MgCl2, 0.32 mM PPi, reaction volume of 0.5 ml)This product is distributed for laboratory research only and not for diagnostic use. |
Storage Buffer | 20 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 8.0 at 25°C. |
Note | This product is distributed for laboratory research only and not for diagnostic use. |